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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #412750
Research Project: Optimizing Nutrient Management and Efficiency of Beef Cattle and Swine

Location: Nutrition, Growth and Physiology

Title: Tissue-specific impact of one-carbon metabolite supplementation and nutrient restriction in gestating heifers on fetal mitochondrial-related gene profiles

Author
item SAFAIN, KAZI - North Dakota State University
item HIRCHERT, MARA - North Dakota State University
item ANAS, MUHAMMAD - North Dakota State University
item ENTZIE, YSSI - North Dakota State University
item SYRING, JESSICA - North Dakota State University
item WARD, ALISON - University Of Saskatchewan
item Crouse, Matthew
item DAHLEN, CARL - North Dakota State University
item CATON, JOEL - North Dakota State University
item SWANSON, KENDALL - North Dakota State University

Submitted to: Current Developments in Nutrition
Publication Type: Abstract Only
Publication Acceptance Date: 3/11/2024
Publication Date: 7/1/2024
Citation: Safain, K.S., Hirchert, M.R., Anas, M., Entzie, Y.L., Syring, J.G., Ward, A.K., Crouse, M.S., Dahlen, C.R., Caton, J.S., Swanson, K.C. 2024. Tissue-specific impact of one-carbon metabolite supplementation and nutrient restriction in gestating heifers on fetal mitochondrial-related gene profiles [abstract]. Current Developments in Nutrition. 8(Supplement 2):283. https://doi.org/10.1016/j.cdnut.2024.102827.
DOI: https://doi.org/10.1016/j.cdnut.2024.102827

Interpretive Summary:

Technical Abstract: Objectives: The study investigated the effects of nutrient restriction and one-carbon metabolite (OCM) supplementation on mitochondrial-related gene expression in fetal liver and muscle of pregnant heifers on day 161 of gestation. Methods: Twenty-nine crossbred Angus heifers, artificially inseminated, were assigned to one of four nutritional treatments using a 2 x 2 factorial design. The first factor, gain, compared a control diet (CON; 0.45 kg/day ADG) against a restricted diet (RES; -0.23 kg/day). The second factor, OCM supplementation, included either a supplemented group (+OCM; methionine [7.4 g/day] and ruminal protected choline [44.4 g/day] in a ground corn carrier, and injections of 20 mg vitamin B12 and 320 mg folate weekly) or a non-supplemented group (-OCM; receiving saline injections and corn carrier). Fetal liver and muscle tissues were collected at 161 days of gestation for RNA extraction and transcriptomic analysis of mitochondrial-related differentially expressed genes (mtDEGs), followed by Gene Ontology (GO) and KEGG pathway analysis. Results: Muscle tissues had more mtDEGs (90%) compared to hepatic tissues (10%), with the majority of muscle mtDEGs being downregulated (FDR P