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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #410364

Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Genetic mapping of resistance QTLs to bacterial leaf streak in wheat

item ACHARYA, KRISHNA - North Dakota State University
item GREEN, ANDREW - North Dakota State University
item LIU, ZHAOHUL - North Dakota State University
item SCHACHTERLE, JEFFREY - Brigham Young University
item KUMARI, POOJA - North Dakota State University
item MANAN, FAZAL - North Dakota State University
item Faris, Justin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/2023
Publication Date: 1/12/2024
Citation: Acharya, K., Green, A., Liu, Z., Schachterle, J., Kumari, P., Manan, F., Faris, J.D. 2024. Genetic mapping of resistance QTLs to bacterial leaf streak in wheat. Meeting Abstract. Poster No. PO0389.

Interpretive Summary:

Technical Abstract: Bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa (Xtu), is an important foliar disease of wheat worldwide and can cause significant yield loss. High levels of BLS resistance are extremely rare in hexaploid wheat, but the hard red spring wheat variety ‘Boost’ has been observed to have good levels of BLS resistance. Here, we evaluated Boost along with other wheat lines for reaction to BLS and found that the synthetic hexaploid wheat line W-7984 is also highly resistant to BLS. To characterize the genetic basis of BLS resistance in Boost and W-7984, we evaluated a recombinant inbred line (RIL) mapping population derived from Boost x ND830 (BLS susceptible) consisting of 190 lines (BoostND population) and the extensively characterized International Triticeae Mapping Population (ITMI population) consisting of 114 RILs derived from W-7984 x Opata 85 for BLS resistance in multiple greenhouse experiments and one field season. QTL analysis of greenhouse experiments using the BoostND population revealed a total of three major QTLs on chromosomes 3B, 5A, and 5B. The 3B QTL was the most significant and explained as much as 38% of the phenotypic variation. For greenhouse experiments on the ITMI population, a QTL with major effects on chromosome 7D was identified that explained as much as 46% of the variation. Both populations were grown in the field in the summer of 2023 and evaluated for BLS under artificial inoculation conditions to determine if the same QTLs were expressed under field conditions. For the BoostND population, the 3B and 5A QTLs were both significantly associated with BLS resistance, but the 5A QTL had stronger effects explaining up to 29% of the variation under field conditions. The 7D QTL in the ITMI population was also significant in the field where it explained 18% of the variation. The effects of the BoostND QTLs were largely additive. We are currently in the process of developing populations to ‘Mendelize’ the major-effect QTLs for fine mapping and cloning of resistance genes/loci and developing lines to combine the 7D QTL from W-7984 with those identified in Boost. The results obtained from this study will provide a means for further understanding BLS resistance and help in developing molecular markers for efficient selection of resistance alleles in wheat breeding programs.