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ARS Home » Southeast Area » Stoneville, Mississippi » Southern Insect Management Research » Research » Publications at this Location » Publication #404585
Research Project: Insect Control and Resistance Management in Corn, Cotton, Sorghum, Soybean, and Sweet Potato, and Alternative Approaches to Tarnished Plant Bug Control in the Southern United States

Location: Southern Insect Management Research

Title: Resistance risk assessment of six pyrethroids and acephate toward the resistant adult tarnished plant bug, Lygus lineolaris (Palisot de Beauvois)

Author
item Du, Yuzhe - Cathy
item Scheibener, Shane
item Zhu, Yu Cheng
item Portilla, Maribel
item Zhang, Minling

Submitted to: Insect Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/31/2023
Publication Date: 10/18/2023
Citation: Du, Y., Scheibener, S.A., Zhu, Y., Portilla, M., Zhang, M(2023)Resistance risk assessment of six pyrethroids and acephate toward the resistant adult tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). Insect Science. https://doi.org/10.1111/1744-7917.13280.
DOI: https://doi.org/10.1111/1744-7917.13280

Interpretive Summary: Due to rapidly developed resistance, pyrethroids are no longer recommended to control economically damaging infestations of the tarnished plant bug (TPB) in cotton fields of Mississippi, but pyrethroid resistance is still prevalent in TPB populations. The study evaluated the resistance levels of TPB to six common pyrethroids and acephate. TBPs were collected from wild host plants in late October after harvest in Mississippi Delta, USA. Our bioassay results showed that the resistant TPBs exhibited higher resistance to permethrin, esfenvalerate and bifenthrin, up to 30~fold, and moderate resistance to '-cyhalothrin, ß-cyfluthrin, '-cypermethrin and acephate, up to 15~fold. Further investigation showed that detoxification activities were significantly increased in resistant TPB. Our finding indicated it is important to avoid early-season pyrethroid sprays , and to rotate neoniconoids (pre-bloom) and sulfoxaflor (mid-season) into current spray regimes to minimize the development of resistance. Additionally, alternative control methods such as biological control and cultural practices are being explored. Overall, this study provides valuable information for the continued use of pyrethroids and acephate in controlling TPBs in cotton fields.

Technical Abstract: Due to rapidly developed resistance, the pesticide management relies less on pyrethroids to control economically damaging infestations of the tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) in cotton fields of Mississippi, yet pyrethroid resistance is still prevalent in TPB populations. In the study, the resistance levels of TPB were evaluated when exposed to six common pyrethroids and acephate. Resistant TBPs were collected from wild host plants in late October after harvest in Mississippi Delta, USA. Based on LC50 values, the resistant TPBs exhibited higher resistance to permethrin, esfenvalerate and bifenthrin (about 30~fold), and moderate resistance to '-cyhalothrin, ß-cyfluthrin, '-cypermethrin and acephate (about 15~fold). Further investigation showed that the inhibitors of general esterase, glutathione S-transferase (GST) and cytochrome P450-monooxygenases (P450), triphenyl phosphate (TPP), diethyl maleate (DEM) and piperonyl butoxide (PBO) had synergistic effects on permethrin, '-cyhalothrin and bifenthrin in resistant TPB. Additionally, elevated activities of general esterase, GST and P450 activities were significantly expressed in resistant TPB, while GST and general esterase were reduced after exposure (~LC50 dose) to some pyrethroids. The synergistic and biochemical assays consistently indicated that P450 and general easterase were involved in pyrethroid detoxification in TPBs. This study provides valuable information for the continued use of pyrethroids and acephate in controlling TPBs in cotton fields.