|KARIITHI, HENRY - Orise Fellow|
|VOLKENING, JEREMY - Base2bio|
|ALVES, VICTORIA - Federal University Of Minas Gerais|
|REIS-CUNHA, JOAO - University Of York|
|ARANTES, LETICIA - Federal University Of Minas Gerais|
|FERNANDO, FILIPE - Boehringer Ingelheim|
|FILHO, TOBIAS - Boehringer Ingelheim|
|MARTINS, NELSON - Federal University Of Minas Gerais|
|LEMIERE, STEPHANE - Boehringer Ingelheim|
|NETO, OLIVEIRO - Federal University Of Minas Gerais|
|DECANINI, EDUARDO - Boehringer Ingelheim|
Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/26/2023
Publication Date: 5/10/2023
Citation: Kariithi, H.M., Volkening, J.D., Alves, V.V., Reis-Cunha, J.L., Arantes, L.C., Fernando, F.S., Filho, T.F., Martins, N.R., Lemiere, S., Neto, O.C., Decanini, E.L., Afonso, C.L., Suarez, D.L. 2023. Complete genome sequences of avian metapneumovirus subtype B vaccine strains from Brazil. Microbiology Resource Announcements. 12(6):e00235-23. https://doi.org/10.1128/JVI.01567-16.
Interpretive Summary: The use of Next Generation Sequencing (NGS) techniques continue to provide a useful way to determine the full sequence of a vaccine or pathogen. Avian metapneumovirus (aMPV) can cause respiratory disease in both chickens and turkeys and is found in both poultry and wild birds. Because of the importance of the virus, vaccination has been used to control clinical disease. This report describes the sequencing of two aMPV vaccine strains from Brazil. The vaccines were similar to each other in sequence and both were classified as subtype B viruses. The submission of these sequences to GenBank allows the public to compare the vaccines to field virus strains.
Technical Abstract: Avian metapneumovirus (aMPV) causes a highly contagious upper respiratory and reproductive disease in chickens, turkeys and ducks. Here, complete genome sequences of live attenuated aMPV subtype B vaccine strains BR/1890/E1/19 (PL21-Nemovac) and BR/1891/E2/19 (1062-Hipraviar) from Brazil were sequenced using next-generation sequencing and compared with the pathogenic field strain VCO3/60616.