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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #400958

Research Project: Improved Conversion of Sugar Crops into Food, Biofuels, Biochemicals, and Bioproducts

Location: Commodity Utilization Research

Title: Metagenome-assembled genomes from sugarcane mill mud

item Uchimiya, Sophie
item ELLIOTT, LEAH - Wells College
item DERITO, CHRISTOPHER - Cornell University
item HAY, ANTHONY - Cornell University

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/6/2023
Publication Date: 10/17/2023
Citation: Uchimiya, M., Elliott, L.E., Derito, C.M., Hay, A.G. 2023. Metagenome-assembled genomes from sugarcane mill mud. Microbiology Resource Announcements. 12(11). Article e00568-23.

Interpretive Summary: It is very difficult to extract and sequence whole community of micro-organisms in soil samples. That is why most studies rely on much easier marker gene (16S) technique. This study, for the first time, successfully sequenced organisms in sugarcane mill mud, which is a byproduct from raw sugar production. Observation of rare organisms in sugarcane biomass indicates its effectiveness as bio-fertilizer and other soil amendments. Benefits of such "sweet" fertilizer and plant growth stimulant could include disease resistance and enhanced crop health.

Technical Abstract: The genomes of 11 bacteria and 3 archaea were assembled from metagenomic DNA extracted from sugarcane mill mud. These Metagenome Assembled Genomes ranged from 1.79 to 6.45'Mb, with 2263 to 5551 proteins, 80.65% to 100% genome completeness, and 43.19% to 68.02% G+C content.