Location: Cereal Crops ResearchTitle: Towards the molecular cloning of tan spot susceptibility gene Tsc2 in wheat
|SINGH, GURMINDER - North Dakota State University|
|RUNNING, KATHERINE - North Dakota State University|
|PETERS HAUGRUD, AMANDA - Oak Ridge Institute For Science And Education (ORISE)|
|SENEVIRATNE, SUDESHI - North Dakota State University|
|SZABO-HEVER, AGNES - Oak Ridge Institute For Science And Education (ORISE)|
|ACHARYA, KRISHNA - North Dakota State University|
|LIU, ZHAOHUI - North Dakota State University|
|DUBCOVSKY, JORGE - University Of California, Davis|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2022
Publication Date: 12/1/2022
Citation: Singh, G., Running, K., Peters Haugrud, A., Seneviratne, S., Zhang, Z., Szabo-Hever, A., Acharya, K., Liu, Z., Dubcovsky, J., Faris, J.D. 2022. Towards the molecular cloning of tan spot susceptibility gene Tsc2 in wheat [abstract]. Plant and Animal Genome 30 Conference. Poster No. PE0550.
Technical Abstract: Tan spot, caused by the necrotrophic fungal pathogen Pyrenophora tritici-repentis, is an important foliar disease that causes significant yield losses in wheat worldwide. The Tsc2 locus on chromosome arm 2BS in wheat recognizes the necrotrophic effector Ptr ToxB to induce a compatible response leading to chlorosis and loss of photosynthetic area on infected wheat leaves, which leads to plant stress and yield loss. In this study, a durum wheat bi-parental population derived from a cross between Kronos (sensitive to Ptr ToxB) and Gredho (insensitive) and a large hard red spring wheat panel were used to delimited the Tsc2 locus to a genetic distance of 1.41 cM, which corresponded to a physical region of 1.37 Mb. The candidate gene region contained 27 putative genes based on the durum wheat reference sequence from Svevo (insensitive to Ptr ToxB). KASP markers were designed to saturate the Tsc2 region using exome capture data from Kronos and Gredho. The molecular markers developed in this study are currently being used for high-resolution mapping of the Tsc2 locus in a large F2 (Kronos × Gredho) population, and candidate genes are being evaluated in several mutant populations. Results from this study will help us clone the Tsc2 gene, gain knowledge and insights regarding the wheat-P. tritici-repentis pathosystem, and develop diagnostic genetic markers to be used in breeding programs to combat tan spot in wheat.