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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #398960
Research Project: Resilient, Sustainable Production Strategies for Low-Input Environments

Location: Crops Pathology and Genetics Research

Title: Investigating grapevine red blotch virus infection in Vitis vinifera L. cv. Cabernet Sauvignon grapes: A multi-omics approach

Author
item Rumbaugh, Arran
item DURBIN-JOHNSON, BLYTHE - University Of California, Davis
item PADHI, EMILY - University Of California, Davis
item LERNO, LARRY - University Of California, Davis
item CAUDURO GIRARDELLO, RAUL - University Of California, Davis
item BRITTON, MONICA - University Of California, Davis
item SLUPSKY, CAROLYN - University Of California, Davis
item Sudarshana, Mysore
item OBERHOLSTER, ANITA - University Of California, Davis

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/27/2022
Publication Date: 10/28/2022
Citation: Rumbaugh, A.C., Durbin-Johnson, B., Padhi, E., Lerno, L., Cauduro Girardello, R., Britton, M., Slupsky, C., Sudarshana, M.R., Oberholster, A. 2022. Investigating grapevine red blotch virus infection in Vitis vinifera L. cv. Cabernet Sauvignon grapes: A multi-omics approach. International Journal of Molecular Sciences. 23(21). Article 13248. https://doi.org/10.3390/ijms232113248.
DOI: https://doi.org/10.3390/ijms232113248

Interpretive Summary:

Technical Abstract: Grapevine red blotch virus (GRBV) is a recently identified virus. Previous research indicates primarily a substantial impact on berry ripening in all varieties studied. The current study analyzed grapes' primary and secondary metabolism across grapevine genotypes and seasons to reveal both conserved and variable impacts to GRBV infection. Vitis vinifera cv. Cabernet Sauvignon (CS) grapevines grafted on two different rootstocks (110R and 420A) were analyzed in 2016 and 2017. Metabolite profiling revealed a considerable impact on amino acid and malate acid levels, volatile aroma compounds derived from the lipoxygenase pathway, and anthocyanins synthesized in the phenylpropanoid pathway. Conserved transcriptional responses to GRBV showed induction of auxin-mediated pathways and photosynthesis with inhibition of transcription and translation processes mainly at harvest. There was an induction of plant-pathogen interactions at pre-veraison, for all genotypes and seasons, except for CS 110R in 2017. Lastly, differential co-expression analysis revealed a transcriptional shift from metabolic synthesis and energy metabolism to transcription and translation processes associated with a virus-induced gene silencing transcript. This plant-derived defense response transcript was only significantly upregulated at veraison for all genotypes and seasons, suggesting a phenological association with disease expression and plant immune responses.