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Research Project: Intervention Strategies to Respond, Control, and Eradicate Foot-and-Mouth Disease Virus (FMDV)

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Title: Foot-and-mouth disease virus SAT2 sequences purified from co-infected Cape buffalo in Kenya

item PALINSKI, RACHEL - Kansas State University
item SANGULA, ABRAHAM - Kenya Fmd Laboratory- Wrlfmd
item GAKUYA, FRANCIS - Wildlife Research And Training Institute
item Bertram, Miranda
item PAUSZEK, STEVEN - Animal And Plant Health Inspection Service (APHIS)
item Hartwig, Ethan
item Smoliga, George
item OBANDA, VINCENT - Wildlife Research And Training Institute
item VANDERWAAL, KIMBERLY - University Of Minnesota
item Arzt, Jonathan
item OMONDI, GEORGE - University Of Minnesota

Submitted to: Microbiology Resource Announcements
Publication Type: Other
Publication Acceptance Date: 8/18/2022
Publication Date: 9/12/2022
Citation: Palinski, R., Sangula, A., Gakuya, F., Bertram, M.R., Pauszek, S., Hartwig, E.J., Smoliga, G.R., Obanda, V., Vanderwaal, K., Arzt, J., Omondi, G. 2022. Foot-and-mouth disease virus SAT2 sequences purified from co-infected Cape buffalo in Kenya. Microbiology Resource Announcements.

Interpretive Summary: Foot-and-mouth disease virus (FMDV) is an important viral disease affecting animal health and economics through impacts on agriculture and trade. African Cape Buffalo are a reservoir for FMD, and can be infected with multiple serotypes without having clinical signs of disease. This paper reports serotype SAT2 sequences from subclinically infected (asymptomatic) buffalo in Kenya. Additionally, we report SAT2 sequences acquired through plaque purification of samples from buffalo that were infected with both SAT1 and SAT2. Plaque purification separates the different viruses in a sample, resulting in more accurate sequences than can be obtained by directly sequencing a sample containing multiple viruses.

Technical Abstract: Foot-and-mouth disease virus SAT2 sequences were acquired from Cape buffalo in Kenya in 2016, from either primary passage (n=38) or plaque purification of SAT1/SAT2 dual-infected samples (n=61). All samples were derived from asymptomatic animals. These genomes contribute to our understanding of FMDV diversity in reservoirs and during sub-clinical FMDV infections.