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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition, Growth and Physiology » Research » Publications at this Location » Publication #393418
Research Project: Improve Nutrient Management and Efficiency of Beef Cattle and Swine

Location: Nutrition, Growth and Physiology

Title: Supplementing one-carbon metabolites to nutrient-restricted cows during early pregnancy affects placental vascularity

Author
item KANJANARUCH, C - Khon Kaen University
item BOCHANTIN, K - North Dakota State University
item BOROWICZ, P - North Dakota State University
item REYNOLDS, L - North Dakota State University
item Crouse, Matthew
item CATON, J - North Dakota State University
item DAHLEN, C - North Dakota State University
item WARD, A - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/22/2022
Publication Date: 8/28/2022
Citation: Kanjanaruch, C., Bochantin, K.A., Borowicz, P.P., Reynolds, L.P., Crouse, M.S., Caton, J.S., Dahlen, C.R., Ward, A.K. 2022. Supplementing one-carbon metabolites to nutrient-restricted cows during early pregnancy affects placental vascularity [abstract]. 2022 Perinatal Biology Symposium. Abstract I-XII (Scientific Program Booklet, p. 27). Available: https://www.asas.org/meetings/perinatal-biology-symposium-2022/scientific-program-(draft)

Interpretive Summary:

Technical Abstract: Objectives: The objectives were to evaluate the effects of plane of nutrition and supplementation of one-carbon metabolites (OCM) on placental vascularity during early gestation. Methods: Thirty-two Angus-crossbred heifers were estrus-synchronized and bred to a single sire with female-sexed semen. The experimental design was a 2 × 2 factorial with two levels of weight gain: control (CON; 0.6 kg/d average daily gain) and restricted (RES; -0.23 kg/d); and OCM: supplementation (+OCM; fed ruminal-protected methionine (10 g/d) and choline (60 g/d) and weekly injections of 320 mg folate and 20 mg vitamin B12) or no supplementation (-OCM; corn carrier and saline injections). Heifers were individually fed and randomly assigned to treatment at breeding (d 0). Placentas were collected on d 63 of gestation (full gestation approximately 280 d). Vascularity was assessed via intensity of CD34 and CD31 fluorescent staining. Images were analyzed for capillary area density (CAD) and capillary number density (CND). Areas evaluated included fetal cotyledon (COT), maternal caruncle (CAR), whole placentome (CAR+COT), fetal membranes (FM), endometrial stratum compactum (SCOM), and endometrial glands (EG). Data were analyzed with the GLM procedure in SAS, with heifer as experimental unit. Results: There was a gain × OCM interaction (P < 0.03) for CAD within CAR+COT and SCOM. Additionally, CAD within FM tended (P = 0.10) to be influenced by gain, while EG was influenced (P = 0.01) by OCM. For CND, there was a tendency (P < 0.10) for COT to be influenced by gain, and CAR and EG by OCM. The CAD was greater in CAR+COT regions of the CON-OCM compared with CON+OCM and RES-OCM (P < 0.02), but not different from RES+OCM. Conclusions: The results indicate that heifer rate of gain and OCM supplementation affected placental vascularization, which may impact placental function during early gestation. (Supported by USDA-NIFA-AFRI 2018-07055).