Location: Aquatic Animal Health ResearchTitle: Shrimp transcriptome analysis after exposure to recombinant Vibrio parahaemolyticus PirA and PirB toxins
Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Database / Dataset
Publication Acceptance Date: 4/4/2022
Publication Date: 12/19/2022
Citation: Rawles, A.A., Abernathy, J.W., Lange, M.D., Shoemaker, C.A., Zhang, D., Bader, T.J., Beck, B.H. 2022. Shrimp transcriptome analysis after exposure to recombinant Vibrio parahaemolyticus PirA and PirB toxins. National Center for Biotechnology Information (NCBI).
Technical Abstract: Vibrio parahaemolyticus is a Gram-negative bacterium commonly found in marine and estuarine environments. Acute hepatopancreatic necrosis disease (AHPND) caused by this bacterium is an ongoing problem among shrimp farming industries. V. parahaemolyticus proteins PirA and PirB have been determined to be major virulence factors that induce AHPND. In this study, Pacific white shrimp (Litopenaeus vannamei) were challenged with recombinant PirA and PirB by a reverse gavage method and then at 30 m, 1, 2, 4, and 6 h time points post infection, the hepatopancreas of five individual shrimp were removed and placed into RNAlater. We conducted RNA sequencing of the hepatopancreas samples from a no PirA/B control (n = 5) and PirA/B-treated shrimp at the different time intervals (n=5). We evaluated the different gene expression patterns between the time groups to the control with a focus on identifying differences in innate immune function. Raw sequencing reads and processed gene expression data has been submitted to the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and is available to the public under the accession number GSE200137.