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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #392698

Research Project: Efficient and Effective Preservation and Management of Plant and Microbial Genetic Resource Collections

Location: Agricultural Genetic Resources Preservation Research

Title: A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agents

item KRECKEL, H - US Department Of Agriculture (USDA)
item Bonnart, Remi
item STICH, D - University Of Colorado
item Volk, Gayle
item LEVINGER, N - Colorado State University

Submitted to: Cryobiology
Publication Type: Abstract Only
Publication Acceptance Date: 5/16/2022
Publication Date: 12/15/2022
Citation: Kreckel, H.D., Bonnart, R.M., Stich, D., Volk, G.M., Levinger, N.E. 2022. A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agents [abstract]. Cryobiology. 109:14-15.

Interpretive Summary: N/A

Technical Abstract: Preserving global plant genetic diversity has never been more important as the number of endangered or at-risk plant species continues to rise worldwide. Vegetatively propagated crop collections have been successfully cryopreserved as 1-2 mm shoot tips treated with highly concentrated mixtures of cryoprotective agents (CPAs) such as PVS2 (15% w/v dimethyl sulfoxide, 15% w/v ethylene glycol, 30% w/v glycerol and 3 M sucrose) prior to plunging them into liquid nitrogen. Despite the successful use of these CPAs, little is understood as to how they protect plant cells and tissues from freezing damage during the cryopreservation process. Coherent anti-Stokes Raman scattering (CARS) microscopy is a vibrational imaging technique that allows for the direct visualization of CPAs within living plant cells and tissues by probing specific chemical bonds. By pairing CARS with brightfield and two-photon endogenous fluorescence microscopies, a comprehensive view of the CPA localization within complex tissue systems can be explored. We describe a multi-modal approach to investigate how Mentha piperita (peppermint) shoot tips respond to dimethyl sulfoxide.