Location: Agricultural Genetic Resources Preservation Research
Title: A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agentsAuthor
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KRECKEL, H - US Department Of Agriculture (USDA) |
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Bonnart, Remi |
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STICH, D - University Of Colorado |
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Volk, Gayle |
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LEVINGER, N - Colorado State University |
Submitted to: Cryobiology
Publication Type: Abstract Only Publication Acceptance Date: 5/16/2022 Publication Date: 12/15/2022 Citation: Kreckel, H.D., Bonnart, R.M., Stich, D., Volk, G.M., Levinger, N.E. 2022. A multi-modal analysis of Mentha x piperita shoot tip response to cryoprotective agents [abstract]. Cryobiology. 109:14-15. https://doi.org/10.1016/j.cryobiol.2022.11.047. DOI: https://doi.org/10.1016/j.cryobiol.2022.11.047 Interpretive Summary: N/A Technical Abstract: Preserving global plant genetic diversity has never been more important as the number of endangered or at-risk plant species continues to rise worldwide. Vegetatively propagated crop collections have been successfully cryopreserved as 1-2 mm shoot tips treated with highly concentrated mixtures of cryoprotective agents (CPAs) such as PVS2 (15% w/v dimethyl sulfoxide, 15% w/v ethylene glycol, 30% w/v glycerol and 3 M sucrose) prior to plunging them into liquid nitrogen. Despite the successful use of these CPAs, little is understood as to how they protect plant cells and tissues from freezing damage during the cryopreservation process. Coherent anti-Stokes Raman scattering (CARS) microscopy is a vibrational imaging technique that allows for the direct visualization of CPAs within living plant cells and tissues by probing specific chemical bonds. By pairing CARS with brightfield and two-photon endogenous fluorescence microscopies, a comprehensive view of the CPA localization within complex tissue systems can be explored. We describe a multi-modal approach to investigate how Mentha piperita (peppermint) shoot tips respond to dimethyl sulfoxide. |