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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » People & Locations » Remi Bonnart

Remi Bonnart
Agricultural Genetic Resources Preservation Research
Biological Science Technician (Plants)

Phone: (970) 492-7613
Fax: (970) 492-7605

1111 South Mason Street
NCGRP
FORT COLLINS, CO 805214500

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Considerations for large-scale implementation of dormant budwood cryopreservation Reprint Icon - (Peer Reviewed Journal)
Tanner, J.D., Chen, K.Y., Bonnart, R.M., Minas, I.S., Volk, G.M. 2020. Considerations for large-scale implementation of dormant budwood cryopreservation. Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240-020-01884-5.
Challenges in implementing plant shoot tip cryopreservation technologies Reprint Icon - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Volk, G.M. 2020. Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240-020-01846-x.
Citrus micrografting for regrowth after shoot tip cryopreservation ()
Volk, G.M., Bonnart, R., Shepherd, A. 2020. Citrus micrografting for regrowth after shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-micrografting/.
Prunus shoot tip cryopreservation ()
Volk, G.M., Bonnart, R. 2020. Prunus shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/prunus-cryopreservation/.
Citrus shoot tip cryopreservation ()
Volk, G.M., Krueger, R., Moreland, B., Bonnart, R., Shepherd, A. 2020. Citrus shoot tip cryopreservation. In: Volk, G.M., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/citrus-cryopreservation/.
Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques Reprint Icon ()
Bettoni JC, Pathirana R, Bonnart R, Shepherd A, Volk G. 2019. Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques. In: Faisal M, Alatar AA, editors. Synthetic Seeds. Switzerland AG: Springer Nature p. 469-482.
Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants Reprint Icon - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Successful cryopreservation and histological observations of Vitis shoot tips isolated from growth chamber source plants. Vitis. 58(2):71-78. https://doi.org/10.5073/vitis.2019.58.71-78.
Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro. HortScience. 54(6):976-981.
Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro Reprint Icon - (Peer Reviewed Journal)
Development of practical and successful Vitis shoot tip cryopreservation protocols - (Abstract Only)
Volk G. 2019. Development of practical and successful Vitis shoot tip cryopreservation protocols. Cryobiology 91:194-195.
Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A.A., Volk, G.M. 2019. Modifications to a Vitis shoot tip cryopreservation procedure: Effect of shoot tip size and use of cryoplates. CryoLetters. 40(2):103-112.
Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants Reprint Icon - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants. Acta Horticulturae. 1234:211-218. https://doi.org/10.17660/ActaHortic.2019.1234.28.
Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2019. Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system. Acta Horticulturae. 1234:329-334. https://doi.org/10.17660/ActaHortic.2019.1234.43.
Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Leado, A., Ayala Silva, T. 2019. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Acta Horticulturae. 1234:335-342. https://doi.org/10.17660/ActaHortic.2019.1234.44.
Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species - (Peer Reviewed Journal)
Volk, G.M., Shepherd, A.N., Bonnart, R.M. 2018. Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species. CryoLetters. 39(5):322-330.
The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil - (Abstract Only)
Bettoni JC, Bonnart R, Shepherd A, Kretzschmar AA, Volk GM. 2018. The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil. Third International Symposium on Plant Cryopreservation. CryoSymp 2018. 26-28 March 2018. Bangkok, Thailand. pg. 24.
Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System - (Abstract Only)
Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2018. Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System. Meeting Abstract. 20.
Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation - (Abstract Only)
Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Ledo, A., Ayala Silva, T. 2018. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Meeting Abstract. 57.
Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips - (Peer Reviewed Journal)
Gross, B.L., Henk, A.D., Bonnart, R.M., Volk, G.M. 2016. Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips. Plant Cell Reports. 36:459-470. doi:10.1007/s00299-016-2095-7.
Citrus cyopreservation: viability of diverse taxa and histological observations - (Peer Reviewed Journal)
Volk, G.M., Bonnart, R.M., Shepherd, A.N., Yin, Z., Lee, R.F., Polek, M., Krueger, R. 2016. Citrus cyopreservation: viability of diverse taxa and histological observations. Plant Cell Tissue and Organ Culture. doi:10.1007/211240-016-1112-4.
Cryopreservation of citrus for long-term conservation - (Peer Reviewed Journal)
Volk, G.M., Bonnart, R.M., Shepherd, A., Krueger, R., Lee, R.F. 2015. Cryopreservation of citrus for long-term conservation. Acta Horticulturae. 1065:187-191.
Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting - (Peer Reviewed Journal)
Bonnart, R.M., Waddell, J.W., Haiby, K., Widrlechner, M., Volk, G.M. 2014. Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting. CryoLetters. 35(6):507-515.
Strategies for improved efficiency when implementing plant vitrification techniques - (Peer Reviewed Journal)
Volk, G.M., Shepherd, A., Bonnart, R.M. 2014. Strategies for improved efficiency when implementing plant vitrification techniques. Acta Horticulturae. 1039:85-89.
Plant shoot tip response to treatment with plant vitrification solution 2 - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Bonnart, R.M., Shepherd, A., Gross, B.L. 2014. Plant shoot tip response to treatment with plant vitrification solution 2. Acta Horticulturae. 1039:81-84.
Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection - (Abstract Only)
Bonnart, R.M., Shepherd, A., Lee, R.F., Krueger, R., Volk, G.M. 2013. Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection. Meeting Abstract. 2nd International Symposium on Plant Cryopreservation. p.62.
Gene expression in arabidopsis shoot tips after liquid nitrogen exposure - (Abstract Only)
Volk, G.M., Henk, A.D., Bonnart, R.M., Gross, B. 2013. Gene expression in arabidopsis shoot tips after liquid nitrogen exposure. Meeting Abstract. 2nd International Society for Horticultural Science Meeting. p.17.
Cryopreservation of vitis shoot tips from diverse species - (Abstract Only)
Shepherd, A., Bonnart, R.M., Volk, G.M. 2013. Cryopreservation of vitis shoot tips from diverse species. Meeting Abstract. Second International Symposium on Plant Cryopreservation, p.103.
Cryopreservation of citrus for long-term conservation - (Abstract Only)
Volk, G.M., Bonnart, R.M., Shepherd, A., Krueger, R., Lee, R.F. 2012. Cryopreservation of citrus for long-term conservation. XII International Citrus Congress, November 18 - 23, 2012, Valencia , Spain. Meeting Abstract. Paper No. S01O02.
Conservation of US citrus collections using cryopreservation - (Abstract Only)
Volk, G.M., Bonnart, R.M., Krueger, R., Lee, R.F. 2012. Conservation of US citrus collections using cryopreservation. Meeting Abstract. Available: http://ashs.org/abstracts/m/abstracts12/abstract_id_10287.html.
Cryopreservation of Citrus shoot tips using micrografting for recovery - (Peer Reviewed Journal)
Volk, G.M., Bonnart, R.M., Krueger, R., Lee, R.F. 2012. Cryopreservation of Citrus shoot tips using micrografting for recovery. CryoLetters. 33(6):418-426.
Increased efficiency using the encapsulation-dehydration cryopreservation technique - (Peer Reviewed Journal)
Bonnart, R.M., Volk, G.M. 2010. Increased efficiency using the encapsulation-dehydration cryopreservation technique. CryoLetters. 31:95-100.
Annonaceae seeds: Desiccation tolerant with unusual physiologies - (Abstract Only)
Volk, G.M., Bonnart, R.M., Walters, C.T. 2009. Annonaceae seeds: Desiccation tolerant with unusual physiologies. Seed Technologists Newsletter. 83 (2):18-19. Association of Official Seed Analysts and Society for Commercial Seed Technologists. May 29-June 4, 2009. Fort Collins, Colorado. Meeting Abstract.
Cryopreservation Of Dormant Buds From Diverse Fraxinus Species - (Peer Reviewed Journal)
Volk, G.M., Bonnart, R.M., Waddell, J.W., Widrlechner, M.P. 2009. Cryopreservation Of Dormant Buds From Diverse Fraxinus Species. CryoLetters. 30:262-267.
High viability of dormant Malus buds after 10 years of storage in liquid nitrogen vapor - (Peer Reviewed Journal)
Volk, G.M., Waddell, J.W., Bonnart, R.M., Towill, L.E., Ellis, D.D., Luffman, M. 2008. High viability of dormant Malus buds after 10 years of storage in liquid nitrogen vapor. CryoLetters 29:89-94.
CRYOPRESERVATION OF ARABIDOPSIS THALIANA SHOOT TIPS. - (Peer Reviewed Journal)
Towill, L.E., R. Bonnart and G.M. Volk. 2006. Cryopreservation of Arabidopsis thaliana shoot tips. CryoLetters 27:353-360.
CRYOPRESERVATION OF APPLE USING NON-DESICCATED SECTIONS FROM WINTER COLLECTED SCIONS - (Peer Reviewed Journal)
Towill, L.E. and R.M. Bonnart. 2005. Cryopreservation of apple using non-desiccated sections from winter collected scions. CryoLetters 26:323-332.
CRACKING IN A VITRIFICATION SOLUTION DURING COOLING OR WARMING DOES NOT EFFECT GROWTH OF CRYOPRESERVED MINT SHOOT TIPS - (Peer Reviewed Journal)
Towill, L.E., Bonnart, R.M. 2003. Cracking in a vitrification solution during cooling or warming does not effect growth of cryopreserved mint shoot tips. CryoLetters. 24:341-346.