Location: Subtropical Plant Pathology ResearchTitle: Complete genome of rice grassy stunt tenuivirus in Thailand and genetic relationship analysis
|SRIVILAI, KANUENGNIJ - Kasetsart University
|WONNAPINIJ, PASSORN - Kasetsart University
|PATARAPUWADOL, SUJIN - Kasetsart University
Submitted to: Thai Journal of Agricultural Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/23/2022
Publication Date: 10/1/2022
Citation: Srivilai, K., Wonnapinij, P., Adkins, S.T., Patarapuwadol, S. 2022. Complete genome of rice grassy stunt tenuivirus in Thailand and genetic relationship analysis. Thai Journal of Agricultural Science. 40(2):114-125. https://doi.org/10.14456/thaidoa-agres.2022.10.
Interpretive Summary: Rice grassy stunt virus (RGSV) causes significant yield reduction to rice in Thailand and other Southeast and East Asian countries. Although this virus was first described in Thailand in 1966, a complete Thai RGSV genome sequence has not been available until a Thai isolate was sequenced in this research project. The genome of the Thai RGSV isolate was ~97% identical to a Philippines RGSV isolate and also closely related to a Chinese isolate. Knowledge of the Thai RGSV genome sequence will help virologists, breeders and regulatory scientists.
Technical Abstract: Total RNA from rice grassy stunt virus (RGSV)-infected rice leaves in Thailand was used to generate a cDNA library for high-throughput sequencing. De novo assembly was used to reconstruct the 25,118 nucleotide genome composed of six RNA segments. Multiple sequence alignments and phylogenetic analyses were used to compare the Thai RGSV isolate with closely related Philippine and Chinese RGSV isolates. This represents the first Thai RGSV genome sequence available to researchers although this virus was first described in Thailand in 1966. The new genome will aid development of improved detection assays and screening rice for resistance to this virus.