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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #389870

Research Project: Genetic Improvement and Cropping Systems of Alfalfa for Livestock Utilization, Environmental Protection and Soil Health

Location: Plant Science Research

Title: Genome sequence resource for strains of Pseudomonas syringae phylogroup 2b and Pseudomonas viridiflava phylogroup 7a causing bacterial stem blight of alfalfa

Author
item LIPPS, SAVANA - University Of Minnesota
item Samac, Deborah - Debby
item ISHII, SATOSHI - University Of Minnesota

Submitted to: Plant Disease
Publication Type: Research Notes
Publication Acceptance Date: 4/13/2022
Publication Date: 8/4/2022
Citation: Lipps, S., Samac, D.A., Ishii, S. 2022. Genome sequence resource for strains of Pseudomonas syringae phylogroup 2b and Pseudomonas viridiflava phylogroup 7a causing bacterial stem blight of alfalfa. Plant Disease. 112:2028-2031. https://doi.org/10.1094/PHYTO-12-21-0511-A.
DOI: https://doi.org/10.1094/PHYTO-12-21-0511-A

Interpretive Summary:

Technical Abstract: Bacterial stem blight (BSB) of alfalfa (Medicago sativa) is a disease characterized by necrotic lesions on stems, chlorosis and necrosis of leaves, and wilt resulting in curled shoot tips resembling a shepherd’s crook, and is associated with ice nucleation active bacteria in the Pseudomonas syringae species complex. Strains grouping with Pseudomonas syringae phylogroup 2b (PG2b) and Pseudomonas viridiflava phylogroup 7a (PG7a) based on citrate synthetase (cts) sequences have been attributed to cause BSB. Here, the genomes of 12 PG2b and eight PG7a strains isolated from alfalfa with symptoms of BSB were sequenced with Illumina NextSeq 550 and Oxford Nanopore Technology (ONT) Minion and were annotated with the NCBI Prokaryotic Genome Annotation Pipeline. These genome sequences will be valuable for further BSB research and may have applications in the development of BSB-resistant alfalfa plants.