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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #387972
Research Project: Human Pathogens within the Produce Production Continuum; their Detection, Mechanisms for Persistence, and Ecology

Location: Produce Safety and Microbiology Research

Title: Complete genome sequence of GII.9 norovirus

Author
item ZHANG, ZILONG - Shanghai International Travel Healthcare Center
item LIU, DANLEI - Shanghai Jiaotong University
item ZHANG, ZILEI - Shanghai Jiaotong University
item Tian, Peng
item LI, S - Shanghai International Travel Healthcare Center
item WU, QINGPING - Shanghai Jiaotong University
item WANG, DAPENG - Shanghai Jiaotong University
item TIAN, ZHENGAN - Shanghai International Travel Healthcare Center

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/24/2021
Publication Date: 10/30/2021
Citation: Zhang, Z., Liu, D., Zhang, Z., Tian, P., Li, S., Wu, Q., Wang, D., Tian, Z. 2021. Complete genome sequence of GII.9 norovirus. Archives of Virology. 167:249-253. https://doi.org/10.1007/s00705-021-05257-x.
DOI: https://doi.org/10.1007/s00705-021-05257-x

Interpretive Summary: Norovirus is recognized as one of the leading causes of acute gastroenteritis outbreaks. Genotype GII.9 was first detected in Norfolk, USA in 1997. However, the complete genome sequence of this genotype was not established yet. In this study, a complete genome sequence of a GII.9[P7] was established by the advanced sequencing method. The complete genome sequence of the virus was 7544 nucleotides (nts). The structure of the viral genome is similar to reported sequences of known noroviral genome. Homology analysis indicated that the virus shares 92.1%-92.3% identity with known GII.P7 noroviruses (AB258331 and AB039777) in nucleotide sequence in RNA dependent polymerase region (p type) and 96.7%-97.4% identity with known GII.9 noroviruses (AY038599 and DQ379715) in amino acid sequences of VP1 (genotyping) . The results suggested that SCD1878 virus could be classfied as a member of GII.P7 for P type and GII.9 for genotype. The viral sequence filled the gap in the whole genome level of the GII.9 genotype.

Technical Abstract: Norovirus is recognized as one of the leading causes of acute gastroenteritis outbreaks. Genotype GII.9 was first detected in Norfolk, USA in 1997. However, the complete genome sequence of this genotype was not established yet. In this study, a complete genome sequence of a GII.9[P7] norovirus, marked as SCD1878, from a patient was established using a highthroughput sequencing and rapid amplification of cDNA ends (RACE) technology. The complete genome sequence of SCD1878_GII.9P7 was 7544 nucleotides (nts) in length with a 3’ poly (A) tail, including three open reading frames. Homology analysis indicated that SCD1878_GII.9P7 shares 92.1%-92.3% identity with GII.P7 (AB258331 and AB039777) and 96.7%-97.4% identity with GII.9 (AY038599 and DQ379715) sequences. The results suggested that SCD1878 is a member of GII.P7 for P genotypes and GII.9 for genotypes. The viral sequence filled the gap in the whole genome level of the GII.9 genotype.