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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #387941

Research Project: Human Pathogens within the Produce Production Continuum; their Detection, Mechanisms for Persistence, and Ecology

Location: Produce Safety and Microbiology Research

Title: A broad-range disposable electrochemical biosensor based on screen-printed carbon electrodes for detection of human noroviruses

Author
item WANG, NAN - Shanghai Jiaotong University
item PAN, GUIYING - Shanghai Jiaotong University
item GUAN, SHIMIN - Shanghai Institute Of Technology
item RONG, SHAOGENG - Shanghai Institute Of Technology
item WANG, DAPENG - Shanghai Jiaotong University
item GAO, ZHIYONG - Beijing Centers For Disease And Prevention, Department Of Pest Inspection
item Tian, Peng
item LI*, QIANQIAN - Shanghai Institute Of Technology

Submitted to: Frontiers in Bioengineering and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/2022
Publication Date: 3/18/2022
Citation: Wang, N., Pan, G., Guan, S., Rong, S., Wang, D., Gao, Z., Tian, P., Li*, Q. 2022. A broad-range disposable electrochemical biosensor based on screen-printed carbon electrodes for detection of human noroviruses. Frontiers in Bioengineering and Biotechnology. 10:845660. https://doi.org/10.3389/fbioe.2022.845660.
DOI: https://doi.org/10.3389/fbioe.2022.845660

Interpretive Summary: Human noroviruses (HuNoVs) are the major non-bacterial pathogen causing acute gastroenteritis in people of all ages worldwide. It is important to develop a rapid detection method for HuNoV. In collaboration with scientists of Shanghai Institute of Technology, an ectrochemical biosensor for sensitive and fast detection of HuNoVs was developed. The electrochemical biosensor targets HuNoVs shell domain proteins which are highly conserved in both GII.4 and GI.1. AuNPs was used for electrical signal enhancement and protein-A was used to linked antibodies in fixed direction. The limit of detection (LOD) of purified recombinant GI.1 viral like particles is 0.37 ng/ml and for GII.4 is 0.22 ng/ml. The LOD of the clinical samples for GII.4 and GI.1 was 104 gene copies/mL. The results could be obtained within an hour. In the study, we demonstrated that the disposable electrochemical biosensor is a good candidate for rapid detection of HuNoVs.

Technical Abstract: Human noroviruses (HuNoVs) are the major non-bacterial pathogen causing acute gastroenteritis in people of all ages worldwide. No stable culture system in vitro was available. The rapid detection of HuNoVs is of great significance for preventing and controlling the pathogens. To address this need, in this work, an electrochemical biosensor for sensitive and fast detection of HuNoVs was fabricated based on screen-printed carbon electrode. The gold nanoparticles on working carbon and protein-A were used to build the biosensor surface. The modified electrode was evaluated by FE-SEM and several electrochemical techniques. The results demonstrated that the detection limitation of GI.1 VP1 was 0.37 ng/mL with a linear range from 1 to 10 ng/mL, and GII.4 VP1 in linear range of 1 to 10 ng/mL with the detection limitation of 0.37ng/mL. Moreover, the detection limitation of the patient fecal samples contaning GII.4 and GI.1 was 104 gene copies/mL. The results could be obtained in 1 hour (from sample to results). It demonstrated that the disposable electrochemical biosensor is a good candidate for rapid detection of HuNoVs.