Location: Ruminant Diseases and Immunology ResearchTitle: Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-a, IL1-ß, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-y bovine IL-17A
|ELNAGGAR, MAHMOUD - Washington State University|
|ABDELLRAZEQ, GABER - Washington State University|
|HULUBEI, VICTORIA - Washington State University|
|DAVIS, WILLIAM - Washington State University|
|HARSLA, TREVOR - Iowa State University|
Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2022
Publication Date: 6/17/2022
Citation: Sacco, R.E., Elnaggar, M.M., Abdellrazeq, G.S., Fry, L.M., Hulubei, V., Davis, W.C., Harsla, T., Mucci, M.L. 2022. Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-a, IL1-ß, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-y bovine IL-17A. Veterinary Immunology and Immunopathology. 250. Article 110456. https://doi.org/10.1016/j.vetimm.2022.110456.
Interpretive Summary: There is a need for the development of tools for the study of the immune systems of veterinary species to further enhance our understanding of comparative animal immunology. In this regard, we have been collaborating on projects to develop tools for the study of white blood cells in veterinary species. We found some of the reagents that we developed for specific ruminant (cattle and sheep) proteins recognize conserved molecules from bottlenose dolphins. To be able to use these tools that are recently developed would be beneficial to examine changes in the immune response of these animals as part of the current effort to monitor the health of bottlenose dolphins and for future studies of their response to vaccination.
Technical Abstract: Opportunities to include Cetancodontamorpha in the study of the evolution of the immune system in the clades of Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have increased with the study of the common bottlenose dolphin, Tursiops truncatus, as a sentinel species to examine the effects of environmental pollutants on the health of marine mammals. Efforts are currently underway to increase the number reagents needed for detailed studies. Thus far, screening of mAbs made to leukocyte differentiation molecules (LDM) and the major histocompatibility (MHC) class I and class II molecules in Ruminantiamorpha have yielded some mAbs that recognize conserved epitopes expressed on orthologues in dolphins. More direct approaches are in progress to identify additional mAbs to dolphin LDM and cytokines. As reported here both direct and indirect approaches were used to identify mAbs specific for cytokines useful in monitoring the effects of environmental pollutants on immune system. Immunization with expressed cytokines yielded mAbs specific for IFN-', TNF-a, IL-6, IL-8, IL-10, and IL-17A. Screening for mAbs that recognize epitopes conserved on dolphin cytokines revealed mAbs developed against ovine IFN-' and bovine IL-17 and IL-1ß recognized epitopes on conserved dolphin orthologues. The mAbs identified in the present study expand the reagents available for study of the function of the immune system in bottlenose dolphins.