Location: Cotton Fiber Bioscience ResearchTitle: Mapping-by-sequencing the locus of EMS-induced mutation responsible for tufted-fuzzless seed phenotype in cotton
Submitted to: Molecular Genetics and Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/2/2021
Publication Date: 6/10/2021
Citation: Naoumkina, M., Thyssen, G.N., Fang, D.D., Bechere, E., Li, P., Florane, C.B. 2021. Mapping-by-sequencing the locus of EMS-induced mutation responsible for tufted-fuzzless seed phenotype in cotton. Molecular Genetics and Genomics. https://doi.org/10.1007/s00438-021-01802-0.
Interpretive Summary: Cotton fiber mutants are useful tools to study cotton fiber development. The n4 is a man-made cotton fiber mutant that has naked seeds with small amount of fuzz (tuft) on both ends of the seed. In this study we developed a large mapping population. We sequenced the genomes of two bulked samples showing the mutant phenotype along with their parents. We have identified the location of n4 mutation on chromosome D04 of cotton genome.Possible candidate genes are discussed in this study. The utilization of the n4 mutant along with other fiber mutants will facilitate our understanding of the molecular mechanisms of cotton fiber cell growth and development.
Technical Abstract: Cotton fiber mutants are valuable resources for studying functions of altered genes and their roles in fiber development. The n4t is a recessive tufted-fuzzless seed mutant created through chemical mutagenesis with ethyl methanesulfonate. Genetic analysis indicated that the tufted-fuzzless phenotype is controlled by a single recessive locus. In this study we developed an F2 population of 602 progeny plants and sequenced the genomes of the parents and two DNA bulks from F2 progenies showing the mutant phenotype. We identified DNA sequence variants between the tufted-fuzzless mutant and wild type by aligning the sequence reads to the reference TM-1 genome and designed subgenome-specific SNP markers. We mapped the n4t locus on chromosome D04 within a genomic interval of about 411 kb. In this region seven genes showed significant differential expression between the tufted-fuzzless mutant and wild type. Possible candidate genes are discussed in this study. The utilization of the n4t mutant along with other fiber mutants will facilitate our understanding of the molecular mechanisms of cotton fiber cell growth and development.