|PARK, SOOK-YOUNG - Suncheon National University|
|KANG, SEOGCHAN - Pennsylvania State University|
|CZYMMEK, KIRK - Danforth Plant Science Center|
Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 7/28/2021
Publication Date: 10/23/2021
Citation: Kim, H.-S., Park, S.-Y., Kang, S., Czymmek, K.L. 2022. Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots. In: Coleman, J., editor. Fusarium wilt. Methods in Molecular Biology, vol 2391. New York, NY: Humana. p. 153-170. https://doi.org/10.1007/978-1-0716-1795-3_13.
Technical Abstract: Microscopic observation of root disease onset and progression is typically performed by harvesting different plants at multiple time points. This approach prevents the monitoring of individual encounter sites over time, often mechanically damages roots, and exposes roots to unnatural conditions during observation. Here, we describe a method developed to avoid these problems and its application to study Fusarium oxysporum-Arabidopsis thaliana interactions. This method enabled the three-dimensional, time-lapse imaging of both A. thaliana and F. oxysporum using confocal and multi-photon microscopy and facilitated inquiries about the genetic mechanism underpinning root pathogenesis.