|GORAICHUK, IRYNA - Consultant|
|DAVIS, JAMES - Georgia Poultry Laboratory Network|
|KULKARNI, ARUN - Georgia Poultry Laboratory Network|
Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/15/2021
Publication Date: 4/15/2021
Citation: Goraichuk, I.V., Davis, J.F., Kulkarni, A.B., Afonso, C.L., Suarez, D.L. 2021. Whole-genome sequence of avian Coronavirus from a preserved 15-year-old sample confirms evidence of GA08 strain circulation 4 years prior to its first reported outbreak. Microbiology Resource Announcements. 10/15:e01460-20. https://doi.org/10.1128/MRA.
Interpretive Summary: Through the use of next generation sequencing methods, we were able to sequence several viruses from clinical samples without the need of growing the viruses in the laboratory. The complete genome of an infectious bronchitis virus (IBV) , an avian coronavirus, was determined from a chicken sample. This virus was closely related to the GA08 strain of IBV that has become an important pathogen of chickens. This sample was collected more than 4 years before the characterization of the GA08 strain which shows it circulated in Georgia earlier than was previously known.
Technical Abstract: Here, we report the complete genome sequence of the Avian coronavirus strain GA08 from a fecal sample of broiler chicken collected in Georgia, USA in 2004. The sequence information of a 15-year-old sample provides evidence for the circulation of the GA08 strain at least 4 years before its first report in 2008.