Dataset of the phospholipidome and transcriptome of Campylobacter jejuni under different growth conditions

Authors: CAO, XUEFENG - Utrecht University; BROUWERS, JOS - Utrecht University; HEIJMEN VAN DIJK, LINDA - Utrecht University; Parker, Craig; Huynh, Steven; VAN PUTTEN, JOS - Utrecht University; KELLY, DAVID - University Of Sheffield; WOSTEN, MARC - Utrecht University

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/21/2020
Publication Date: 9/28/2020
Citation: Cao, X., Brouwers, J., Heijmen Van Dijk, L., Parker, C., Huynh, S., Van Putten, J., Kelly, D.J., Wosten, M.M. 2020. Dataset of the phospholipidome and transcriptome of Campylobacter jejuni under different growth conditions. Data in Brief. 33. Article 106349. https://doi.org/10.1016/j.dib.2020.106349.
DOI: https://doi.org/10.1016/j.dib.2020.106349

Interpretive Summary: The Gram-negative bacterium Campylobacter jejuni is one of the leading causes of bacterial foodborne illness causing approximately 400 million cases of intestinal infection each year. C. jejuni grows best at low O2 and high CO2 levels and an ambient temperature of 42ºC. These conditions are present in the intestinal tract of poultry, which is often colonized by C. jejuni in very high numbers. The membrane phospholipid composition of C. jejuni is not a stable bacterial characteristic but can change in response to altered environmental conditions. Here, we provide the dataset of the phospholipidome and transcriptome of the microaerophilic human pathogen Campylobacter jejuni grown under different environmental conditions. These data have been used in Cao et al (2020), The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysolipids are required for motility at low oxygen availability. The data show the abundance of each phospholipid during the growth of C. jejuni for 0-108 h under low and high oxygen conditions (0.3 vs 10 % O2). The phospholipid data were obtained by applying high performance liquid chromatography tandem-mass spectrometry (LC-MS/MS). The transcriptomic data were obtained by RNA-seq and show the differential expressed genes between logarithmic and stationary grown bacteria. In addition, our data might serve as reference information for further in-depth investigation to understand the relationship between specific phospholipids and the activity of membrane associated proteins.

Technical Abstract: The membrane phospholipid composition is not a stable bacterial characteristic but can change in response to altered environmental conditions. Here we provide the dataset of the phospholipidome and transcriptome of the microaerophilic human pathogen Campylobacter jejuni under different environmental conditions. These data have been used in Cao et al (2020), The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysolipids are required for motility at low oxygen availability. Here the abundance of each phospholipid is shown during the growth of C. jejuni for 0-108 h under low and high oxygen conditions (0.3 vs 10 % O2). The phospholipid data were obtained by applying high performance liquid chromatography tandem-mass spectrometry (LC-MS/MS). The transcriptomic data obtained by RNA-seq show the differential expressed genes between logarithmic and stationary grown bacteria. In addition, our data might serve as a reference information for further in-depth investigation to understand the relationship between specific phospholipids and the activity of membrane associated proteins.