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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #375904

Research Project: Biology, Ecology, and Genomics of Pathogenic and Beneficial Microorganisms of Wheat, Barley, and Biofuel Brassicas

Location: Wheat Health, Genetics, and Quality Research

Title: Genome-wide identification and expression profile analysis of the phospholipase C gene family in wheat (Triticum aestivum L.)

Author
item WANG, XIANGUO - Northwest A&f University
item LIU, YAN - Northwest A&f University
item LI, ZHENG - Northwest A&f University
item GAO, XIANG - Northwest A&f University
item DONG, JIAN - Northwest A&f University
item ZHANG, JIACHENG - Northwest A&f University
item ZHANG, LONGLONG - Northwest A&f University
item Thomashow, Linda
item Weller, David
item YANG, MINGMING - Northwest A&f University

Submitted to: Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/10/2020
Publication Date: 7/13/2020
Citation: Wang, X., Liu, Y., Li, Z., Gao, X., Dong, J., Zhang, J., Zhang, L., Thomashow, L.S., Weller, D.M., Yang, M. 2020. Genome-wide identification and expression profile analysis of the phospholipase C gene family in wheat (Triticum aestivum L.). Plants. 9(7), 885. https://doi.org/10.3390/plants9070885.
DOI: https://doi.org/10.3390/plants9070885

Interpretive Summary: Enzymes that degrade phospholipid compounds include members of the phospholipase C (PLC) family, which play important roles in regulating plant growth and responding to stress. In the present study, computer analysis revealed 26 PLC genes named TaPLCs. Further analyses divided the wheat PLC genes into 2 families, TaPIPLC (containing the typical X, Y and C2 protein regions) and TaNPC (containing a region targeting the phosphatase functional portion of the protein). TaPLC expression patterns differed among tissues and organs of wheat and under environmental stress conditions. The expression levels of 8 TaPLC genes were confirmed through PCR analyses. Most PLC genes in Arabidopsiswere sensitive to salt stress and were rapidly expressed at higher levels, and some were sensitive to low temperatures and drought. Overexpression in the model plant Arabidopsis of the aPI-PLC1-2B gene conferred significant resistance to salt and drought stress, and the primary root of Arabidopsis strain P1-OE was significantly longer than that in the wild type parental plant. Our results extend our knowledge of the PLC gene family in wheat, and lay a solid foundation for further research on their function.

Technical Abstract: Phospholipid-hydrolyzing enzymes include members of the phospholipase C (PLC) family, which play important roles in regulating plant growth and responding to stress. In the present study, a systematic in silico analysis of the wheat PLC gene family revealed a total of 26 wheat PLC genes (TaPLCs). Phylogenetic and sequence alignment analyses divided the wheat PLC genes into 2 clusters, TaPIPLC (containing the typical X, Y and C2 domains) and TaNPC (containing a phosphatase domain). TaPLC expression patterns differed among tissues, organs and under abiotic stress conditions. The transcript levels of 8 TaPLC genes were validated through qPCR analyses. Most of TaPLC genes were sensitive to salt stress and were up-regulated rapidly, and some TaPLC genes were sensitive to low temperatures and drought. Overexpression of TaPI-PLC1-2B had significant resistance to salt and drought stress, and the primary root of P1-OE was significantly longer than the wild type in Arabidopsis. Our results extend our knowledge of the PLC gene family in wheat, and laid a solid foundation for further research on the function of PLC family genes in wheat.