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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #370924
Research Project: Discovery and Development of Natural Products for Pharmaceutical and Agrochemical Applications II

Location: Natural Products Utilization Research

Title: Two new phytoecdysteroids from Sphenocentrum jollyanum Pierre root

Author
item AJAYI, TEMITAYO - University Of Ibadan
item SRIVEDAVYASASRI, RADHAKRISHNAN - University Of Mississippi
item NYONG, EMMANUEL - University Of Ibadan
item ODENIYI, MICHEAL - University Of Ibadan
item MOODY, JONES - University Of Ibadan
item ROSS, SAMIR - University Of Mississippi

Submitted to: Steroids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/9/2019
Publication Date: 7/19/2019
Citation: Ajayi, T.O., Srivedavyasasri, R., Nyong, E.E., Odeniyi, M.A., Moody, J.O., Ross, S.A. 2019. Two new phytoecdysteroids from Sphenocentrum jollyanum Pierre root. Steroids. 150:108456. https://doi.org/10.1016/j.steroids.2019.108456.
DOI: https://doi.org/10.1016/j.steroids.2019.108456

Interpretive Summary: The methanol extract of Sphenocentrum jollyanum root exhibited antimicrobial activity against Aspergillus fumigatus Pinh and Vancomycin resistant enterococcus (VRE) with IC50 11.45 and 12.95 µg/mL, respectively. The ethyl acetate fraction of methanol extract showed activity against A. fumigatus Pinh with IC50 of <8 µg/mL. Phytochemical investigation of ethyl acetate fraction yielded six compounds, identified as two new phytoecdysteroidal glycosides Sphenocentroside A, and Sphenocentroside B, and four known phytoecdysteroids: polypodoaurein, polypodine B, ecdysterone, and 20, 26-dihydroxyecdysone.

Technical Abstract: The crude methanol extract of Sphenocentrum jollyanum root exhibited 98% and 80% antimicrobial activity against Aspergillus fumigatus Pinh and Vancomycin resistant enterococcus (VRE) at a concentration of 200 µg/mL, with IC50 11.45 and 12.95 µg/mL, respectively. The ethyl acetate fraction of methanol extract showed in-vitro antimicrobial activity against A. fumigatus Pinh at 83% with IC50 of <8 µg/mL. The phytochemical investigation of ethyl acetate fraction yielded six compounds, which were identified by their Nuclear Magnetic Resonance (NMR), Infrared Radiation (IR) and Mass Spectrometry (MS) spectral analyses as two new phytoecdysteroidal glycosides Sphenocentroside A (1), and Sphenocentroside B (2), and four known phytoecdysteroids: polypodoaurein (3), polypodine B (4), ecdysterone (5), and 20, 26-dihydroxyecdysone (6).