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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #369895

Research Project: Identification of the Ecological Niches and Development of Intervention Strategies to Reduce Pathogenic Foodborne Pathogens in Poultry

Location: Food and Feed Safety Research

Title: Influence of organic and inorganic acids as used for poultry part dips on the emergence of spoilage organisms during a shelf-life study

item DITTOE, DANA - University Of Arkansas
item Feye, Kristina
item OVALL, CHRISTINA - Jones-Hamilton Co
item KNUEVEN, CARL - Jones-Hamilton Co
item RICKE, STEVEN - University Of Arkansas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/2/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Due to concern over the use of peracetic acid (PAA) on its potential to select for lactic acid producing bacteria (LAB) commonly associated with spoilage, alternatives are being explored. The objective of the current study was to determine the potential of sodium bisulfate (SBS) as an alternative to PAA in poultry part dips during a 21 d shelf-life study. In total, 200 wings (5 treatments, 4 times, 10 replications) were obtained from an Arkansas poultry processor and subjected to the following treatments, which included not treated (NT) or treated with 350 mL of the following 15-sec dips: tap water (TW), TW + PAA (500 ppm), TW + SBS (3%), TW + PAA + SBS. Wings were stored at 4 deg C until microbial analyses. On d 0, 7, 14, and 21, wings were rinsed in 150 mL of nBPW and subsequently plated on MRS and TSA to enumerate total LAB and aerobes. Colonies were log10 transformed and reported as CFU per gram of wing. Data were analyzed in JMP 14.0 as a Randomized Complete Block Design using simple linear regression and one-way ANOVA with means being separated using Tukey’s HSD (P less than or equal to 0.05). Over time, both LAB and aerobes increased, with TW + SBS and TW + SBS + PAA having lower loads of aerobic bacteria and LAB on d 7 and TW + SBS having lower loads of both on d 14 (P less than 0.05). By day, there was no difference between treatments and controls on d 0, but on d 7 and 14 the load of LAB was below 1 log10 (CFU/g) or below the limit of detection for wings treated with TW + SBS and TW + SBS + PAA compared to those treated with the controls and TW + PAA (P less than 0.05). The rinsates were sequenced with Illumina MiSeq 16S rDNA sequencing and processed through QIIME2.2019.8. Data indicates that SBS (3) + PAA induces a unique microbiome that may improve shelf life based on the microbial data as lactic acid-producing bacteria are reduced (Q less than 0.05: ANCOM). Additionally, the stability of the microbiomes of each of the parts over time varies as indicated by MAZ (microbiota-by-age score; P less than 0.05). Therefore, it can be concluded that the use of 3% SBS alone or in combination with PAA has the potential to mitigate the selection of LAB when applied to a short duration part dip over a 14 d shelf life.