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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #368385

Research Project: Integration of Traditional Methods and Novel Molecular Strategies for Improving Disease Resistance and Input-use Efficiency in Peanut

Location: National Peanut Research Laboratory

Title: GWAS and co-expression network reveal ionomic variation in cultivated peanut

Author
item ZHANG, HUI - Auburn University
item Wang, Ming
item SCHAEFER, ROB - University Of Minnesota
item Dang, Phat
item JIANG, TAO - Auburn University
item CHEN, CHARLES - Auburn University

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/7/2019
Publication Date: 10/7/2019
Citation: Zhang, H., Wang, M.L., Schaefer, R., Dang, P.M., Jiang, T., Chen, C. 2019. GWAS and co-expression network reveal ionomic variation in cultivated peanut. Journal of Agricultural and Food Chemistry. 63(43)12026-12036. https://doi.org/10.1021/acs.jafc.9b04939.
DOI: https://doi.org/10.1021/acs.jafc.9b04939

Interpretive Summary: Peanut is an important legume providing products with high nutritional values, including important minerals. Plant ionome is defined as the mineral nutrients and trace elements in plants, and their composition in peanut seed is important for human health. However, the genetic basis underlying the variations in the mineral composition in peanut is still unknown. A genetics-based study called genome-wide association studies (GWAS) linking genetic differences of peanuts to the concentrations of 13 mineral elements using a diverse panel of 120 genotypes mainly selected from the U.S. peanut mini core germplasm collection. A total of 36 significant quantitative trait loci (QTLs) associated with 5 elemental concentrations were identified with high correlations, in which 24 are for boron (B), 2 for copper (Cu), 6 for sodium (Na), 3 for sulfur (S), and 1 for zinc (Zn). A total of 110 candidate genes were identified with high association with elements accumulation and includes one high-priority overlap (HPO) candidate gene, an important elemental/metal transporter gene located on chromosome 4. The identification of the genes responsible the accumulation and transport mineral elements will enable the development of peanut varieties with enhanced important nutrients and minerals.

Technical Abstract: Peanut is an important legume providing products with nutrient dense including mineral nutrition. However, the genetic basis underlying the variations in the mineral composition in peanut is still unknown. A genome-wide association studies (GWAS) of the concentrations of 13 mineral elements combine with co-expression network were performed using a diverse panel of 120 genotypes mainly selected from the U.S. peanut mini core collection grown in two years to study genetic variation in peanut ionome. A total of 36 significant quantitative trait loci (QTLs) associated with 5 elemental concentrations were identified with phenotypic variation explained (PVE) from 18.35% to 27.56%, in which 24 QTLs are for boron (B), 2 QTLs for copper (Cu), 6 QTLs for sodium (Na), 3 QTLs for sulfur (S), and 1 QTL for zinc (Zn). A total of 110 non-redundant candidate causal genes identified were significantly associated with elements accumulation, which include one high-priority overlap (HPO) candidate gene arahy.KQD4NT, an important elemental/metal transporter gene located on LGB04 with position 5,413,913-5,417,353.