Quantitative trait loci for field resistance to barley stripe rust derived from malting line 95SR316A

Authors: Esvelt Klos, Kathy; HAYES, PATRICK - Oregon State University; DEL BLANCO, ISABEL ALICIA - University Of California; Chen, Xianming; FILICHKIN, TANYA - Oregon State University; HELGERSON, LAURA - Oregon State University; FISK, SCOTT - University Of California; Bregitzer, Paul

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/6/2020
Publication Date: 3/17/2020
Citation: Esvelt Klos, K.L., Hayes, P., Del Blanco, I., Chen, X., Filichkin, T., Helgerson, L., Fisk, S., Bregitzer, P.P. 2020. Quantitative trait loci for field resistance to barley stripe rust derived from malting line 95SR316A. Crop Science. 60(4):184-1853. https://doi.org/10.1002/csc2.20154.
DOI: https://doi.org/10.1002/csc2.20154

Interpretive Summary: Adult plant resistance to barley stripe rust disease, governed by multiple genes, is thought to be more durable under field conditions than seedling resistance conferred by one or a few genes. The two-rowed malting barley breeding line "95SR316A" possesses adult plant resistance as well as desirable agronomic traits and good malting quality. Two crosses were made between this and other barley lines in order to map the locations of quantitative trait loci (QTL) contributing to adult plant resistance. Six QTL for barley stripe rust resistance inherited from 95SR316A were mapped. Molecular genetic markers in these regions may be useful to breeders seeking to rapidly transfer these disease resistance QTL into new barley varieties.

Technical Abstract: Developing improved barley (Hordeum vulgare L.) germplasm with resistance to barley stripe rust (BSR) has been a goal of several breeding programs since the introduction of this disease into the Americas. The USDA–ARS two-rowed spring malting barley breeding line 95SR316A, while susceptible at the seedling stage, has field resistance to BSR. This study was conducted to identify markers useful for marker-assisted selection for resistance to BSR. This line was used as a parent in two biparental mapping populations: 95SR316A × ‘Lenetah’ (susceptible), and 95SR316A × ‘Grannelose Zwiezelige’ (GZ; seedling resistance). Progeny were observed for response to BSR infection in the field at four location–years per population. Disease severity was taken as the percentage of leaf area infected. The 95SR316A × GZ (F5–derived recombinant inbred line [RIL]) and 95SR316A × Lenetah (doubled haploid) populations were genotyped using Illumina's 50K barley array. Interval mapping revealed quantitative trait loci (QTL) in both populations on chromosome 2H (Qpsh.316A.2Ha) near the p-terminal, with the allele derived from 95SR316A contributing to lower disease severity. In addition, the 95SR316A × GZ population was segregating for a QTL on chromosome 2H (Qpsh.316A.2Hb) at ~35 cM with effects on BSR observed in two location years. The 95SR316A × Lenetah population was segregating for Qpsh.316A.7H at 94.04 cM on chromosome 7H with peak logarithm of the odds (LOD) scores of 25.81, 36.75, and 5.35 observed in Corvallis, OR, in 2018, Davis, CA, in 2018, and Mt. Vernon, WA, in 2018, respectively. The QTL on chromosomes 3H, 5H, and 6H were observed in only single location–years in the 95SR316A × Lenetah population.