Location: Endemic Poultry Viral Diseases ResearchTitle: Expression of two foreign genes from the optimal insertion sites of Newcastle disease virus vector for use as a multivalent vaccine and gene therapy vector
|HE, LEI - Henan Institute Of Science And Technology|
|ZHANG, ZHENYU - Harbin Veterinary Research Institute|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/8/2020
Publication Date: 2/7/2020
Citation: He, L., Zhang, Z., Yu, Q. 2020. Expression of two foreign genes from the optimal insertion sites of Newcastle disease virus vector for use as a multivalent vaccine and gene therapy vector. Meeting Abstract. https://viruses2020.sciforum.net/conferences_files/265/customs/8e7a8d27a5bbc4414b3bd49b0dfe4dd2.pdf.
Technical Abstract: Newcastle disease virus (NDV) vaccine strains have been developed as vectors to express foreign genes as multivalent vaccine candidates for animals. A majority of these NDV vectors express only a single foreign gene (FG) through either an independent transcription unit (ITU) or an internal ribosomal entry site (IRES) approach. In the present study, we generated NDV LaSota strain-based recombinant viruses to express two FGs, green fluorescent protein (GFP) and red fluorescent protein (RFP) genes, from the identified optimal insertion sites through a combination of the ITU and IRES approaches. Biological assessments of these recombinant viruses showed that the insertion of FGs slightly attenuated the virus pathogenicity, yet the growth kinetics and viral yields in vitro were comparable with those of the parental rLS strain. Comparisons of the fluorescence intensities of GFP and RFP expressed from virus-infected DF-1 cells revealed that the levels of GFP and RFP expressed by the viruses vectoring two FGs were comparable to those expressed by the viruses vectoring only a single FG under the same expression approach. The expression of FGs through the ITU approach was approximately 4-fold more efficient than that through the IRES approach. These results suggest that the NDV LaSota vector is a safe and promising multivalent vaccine vector. The ITU approach could be used for expression of a higher amount of FG products, whereas the IRES approach for expression of a lower amount of FG products to meet the requirement for vaccine or gene therapy purposes.