Location: Endemic Poultry Viral Diseases ResearchTitle: ALV-miRNA-p19-01 promotes viral replication via targeting dual specificity Phosphatase 6
|YAN, YIMING - South China Agricultural University|
|CHEN, SHENG - South China Agricultural University|
|LIAO, SHUANG - South China Agricultural Univerisity|
|GAO, SHUANG - Guangdong Academy Of Agricultural Sciences|
|PANG, YANLING - South China Agricultural Univerisity|
|ZHANG, XINHENG - South China Agricultural University|
|XIE, QINGMEI - South China Agricultural University|
Submitted to: Viruses
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2022
Publication Date: 4/13/2022
Citation: Yan, Y., Chen, S., Liao, S., Gao, S., Pang, Y., Zhang, X., Zhang, H., Xie, Q. 2022. ALV-miRNA-p19-01 promotes viral replication via targeting dual specificity Phosphatase 6. Viruses. https://doi.org/10.3390/v14040805.
Interpretive Summary: MicroRNAs are a small classes of RNA molecules, differing from relatively large RNAs, that do not produce any protein products. But microRNAs are richly documented in regulatory rolls controlling gene expression and large RNA products. One such microRNA was identified in this study, which was originated from one subgroup of avian leukosis virus. Further laboratory examinations showed this microRNA provides functional support in avian leukosis virus infection process. This is the first microRNA identified in this particular subgroup of avian leukosis virus with detected function, which is of importance in better understanding the virus infection mechanism.
Technical Abstract: MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miRNA. Here, we reported a novel miRNA (designated ALV-miRNA-p19-01) in ALV-J-infected DF-1 cells. We found that ALV-miRNA-p19-01 is encoded by the genome of the ALV-J SCAU1903 strain (located at nucleotides site 779 to 801) in a classic miRNA biogenesis manner. The transfection of DF-1 cells with ALV-miRNA-p19-01 enhanced ALV-J replication, while the blockage of ALV-miRNA-p19-01 suppressed ALV-J replication. Furthermore, our data showed that ALV-miRNAp19-01 promotes ALV-J replication by directly targeting the cellular gene dual specificity phosphatase 6 through regulating ERK2 activity.