Location: Plant Polymer ResearchTitle: Preparation and characterization of carboxymethyl cashew gum grafted with immobilized antibody for potential biosensor application
|ARAUJO MELO, AIRIS - Universidade Estadual Do Ceara|
|FELIX OLIVEIRA, MARIA - Universidade Estadual Do Ceara|
|FURTANDO, ROSELAYNE - Embrapa|
|DE FATIMA BORGES, MARIA - Embrapa|
|ALVES, CARLUCIO - Universidade Estadual Do Ceara|
Submitted to: Carbohydrate Polymers
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/29/2019
Publication Date: 9/30/2019
Citation: Araujo Melo, A.M., Felix Oliveira, M.R., Furtando, R.F., de Fatima Borges, M., Biswas, A., Cheng, H.N., Alves, C.R. 2020. Preparation and characterization of carboxymethyl cashew gum grafted with immobilized antibody for potential biosensor application. Carbohydrate Polymers. 228:115408. https://doi.org/10.1016/J.carbpol.2019.115408.
Interpretive Summary: This work shows that cashew gum derivatives can provide a versatile platform to immobilize selected biomolecules that can function as bioreceptors in the future development of novel bio-devices in food and biomedical applications. Cashew gum is a by-product of the cashew industry and has little commercial value. The possibility of using cashew gum as a biosensor for bacteria detection would be a welcome development for the industry.
Technical Abstract: This report details the design of carboxymethylated cashew gum (CG) as a platform for antibody (Ab) immobilization, which can then be used as a biosensor for bacteria detection. The CG was isolated and characterized, followed by conversion to carboxymethyl cashew gum (CMCG). The CMCG film was a viable support for antibody immobilization; it was electrodeposited on gold surface using the cyclic voltammetry technique, applying a potential sweep from -1.0 V to 1.3 V with a scan rate of 50 mV s-1 and 10 scans. The COOH groups on the surface of the film were critical in promoting Ab bonding. The immobilization of the Ab was mediated by protein A (PrA) for recognition of the antigen. Voltammetry studies were used to monitor the antibody immobilization. Finally, the analytical response of the CMCG-PrA-Ab system was evaluated with the chronoamperometry technique and was found to detect Salmonella Typhimurium bacteria rapidly and efficiently.