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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #366259

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Biological properties and genomic sequence of an isolate of Cherry rasp leaf virus from tomato

item BRATSCH, SARA - University Of Minnesota
item Grinstead, Sam
item LOCKHART, BEN - University Of Minnesota
item Mollov, Dimitre

Submitted to: Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/3/2020
Publication Date: 2/24/2020
Citation: Bratsch, S., Grinstead, S.C., Lockhart, B., Mollov, D.S. 2020. Biological properties and genomic sequence of an isolate of Cherry rasp leaf virus from tomato. Journal of Plant Pathology.

Interpretive Summary: Tomato is a valuable vegetable crop grown in the U.S. In 2016 tomatoes grown in high tunnels from Minnesota exhibited virus like symptoms. Virus particles purified from infected tomatoes were used to inoculate the tobacco bioassay plant N. benthamiana. The inoculated plants developed symptoms and subsequent tests confirmed they were caused by a virus. The virus was identified as cherry rasp leaf virus (CRLV). The complete genomic sequence of this CRLV isolate from tomato was determined. Knowing the CLRV genome sequence facilitates developing reliable detection methods and better control measures of this virus. This is the first report of CRLV infecting tomatoes, and also the first report of this virus in Minnesota. This information will be useful to the vegetable industry, plant diagnosticians and regulatory agencies.

Technical Abstract: Cherry rasp leaf virus (CRLV) is found in the USA infecting Prunus and Malus spp. In 2016, symptomatic high tunnel grown tomatoes (Solanum lycopersicum L.) from Minnesota exhibiting viral symptoms were found to contain ~30 nm spherical particles. High throughput sequencing (HTS) revealed two distinct viral contigs, attributed to RNA 1 (complete, MK952187) and RNA 2 (5’ partial, MK952188) of a new CRLV isolate. The isolate, denoted CRLV-tom, shared 87-98% nucleotide identity with three previously sequenced isolates of CRLV. CRLV-tom was mechanically inoculated into Nicotiana benthamiana and caused symptoms of interveinal necrotic line patterns three weeks after inoculation. Symptoms progressed into leaf rugosity and chlorosis ten weeks after inoculation. Inoculated greenhouse tomatoes were infected by CRLV, as detected by RT-PCR, but were asymptomatic. This is the first report of CRLV infecting tomato and of CRLV in Minnesota.