|KOBAYASHI, NAOKI - Azabu University|
|UCHIYAMA, YOSUKE - Azabu University|
|SUGITA-KONISHI, YOSHIKO - Azabu University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/16/2019
Publication Date: 10/16/2019
Citation: Maragos, C.M., Kobayashi, N., Uchiyama, Y., Sugita-Konishi, Y. 2019. Development of antibodies for the mycotoxin citreoviridin [abstract].
Technical Abstract: Citreoviridin (CTV) was discovered many years ago as one of the yellow rice toxins. It is produced by a variety of species including Penicillium citreonigrum, Aspergillus terreus, and Eupenicillium ochrosalmoneum. It is known to cause acute toxicity in mice, rats, guinea pigs, and ducklings. One of the sites of action of CTV is mitochondrial ATPase. Intoxication with CTV is marked by ascending paralysis, disturbances of the central nervous system, and respiratory arrest. Consumption of CTV has been associated with acute cardiac beriberi, manifested as neurological symptoms and heart failure. Chronic exposure has been suggested to be a potential trigger of Keshan disease. While it is known primarily for its association with rice, CTV has also been found in corn and pecan nuts. There are very few reports of antibodies that have been developed against this toxin. We have developed and characterized two sensitive monoclonal antibodies for CTV, which were given the shortened designations “2-2” and “2-4”. In competitive antigen-immobilized enzyme-linked immunosorbent assays (CI-ELISAs) in buffer, the observed IC50s were 11 ng/mL and 18 ng/mL for 2-2 and 2-4, respectively. Both antibodies were relatively tolerant to the presence of methanol. When CTV standards were prepared in 20% methanol, the IC50s increased only slightly, to 15 and 21 ng/mL, respectively. The antibodies give indications that they may also perform well at higher methanol concentrations, however methanol levels greater than 30% tended to precipitate the serum albumins added to promote antibody stability. Cross reactivity with three compounds having structural similarity to portions of the CTV molecule was minimal. Results suggest that these antibodies will be sensitive enough to find application in immunoassays and biosensors for CTV in commodities such as rice and corn.