Location: Produce Safety and Microbiology ResearchTitle: Malvidin 3-glucoside modulated gut microbial dysbiosis and global metabolome disrupted in a murine colitis model induced by dextran sulfate sodium
|LIU, FANG - Ocean University Of China|
|Wang, Thomas - Tom|
|TANG, QINGJUAN - Ocean University Of China|
|XUE, CHANGHU - Ocean University Of China|
Submitted to: Molecular Nutrition and Food Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/28/2019
Publication Date: 8/24/2019
Citation: Liu, F., Wang, T.T., Tang, Q., Xue, C., Li, R.W., Wu, V.C. 2019. Malvidin 3-glucoside modulated gut microbial dysbiosis and global metabolome disrupted in a murine colitis model induced by dextran sulfate sodium. Molecular Nutrition and Food Research. 1900455:1-14. https://doi.org/10.1002/mnfr.201900455.
Interpretive Summary: The consumption of blueberries in the US has been growing rapidly due to their potential health benefits. Malvidin is arguably the most important anthocyanin in blueberries. However, mechanisms of malvidin in modulating gut microbiome and metabolome remain unclear. In this study, we characterized the effect of malvidin ingestion on the gut microbial community and metabolism using an experimental colitis mouse model. Our findings suggest that malvidin possessed anti-inflammatory properties by modulating the integrity of the colon epithelium, gut microbiota, and gut metabolism. We also compared the similarities and differences between a single bioactive ingredient and the whole fruit from which the ingredient is derived. Our results should facilitate the development of blueberries as a functional food.
Technical Abstract: The study aimed to understand the mechanisms of the anthocyanin malvidin-3 glucoside (MV) in alleviating gut dysbiosis using a murine colitis model induced by dextran sulfate sodium (DSS). The effect of MV on the structure and function of the colon microbiome and microbial metabolism was evaluated using 16S rRNA gene sequencing, global metabolomics, and a Random-Matrix Theory based network algorithm. MV ingestion improved histopathological scores and increased IL10 expression in colon mucosa of colitis mice. While DSS had a profound effect on the gut microbiome and significantly repressed both microbial richness and evenness, MV further reduced evenness but promoted microbial interactions and restored the Firmicutes to Bacteroidetes ratio repressed by DSS. Moreover, MV reduced the incident of pathogenic bacteria, such as Ruminococcus gnavus, in colitis mice. Furthermore, MV had a strong modulatory effect on microbial co-occurrence patterns and gut metabolites. MV reversed several key inflammatory mediators, including sphingolipid metabolites, from elevated levels in DSS colitis mice. As a bioactive ingredient, MV exerted its effect on the gut microbiome in the mechanism that differed from the whole blueberry. MV ingestion ameliorated intestinal inflammation by modulating colon epithelium integrity, gut microbiome, and key inflammatory mediators.