Location: Endemic Poultry Viral Diseases ResearchTitle: Marek's Disease-vaccines induced differential expression of microRNAs in the primary lymphoid organ of bursa at 26th day post inoculation.
|ZHANG, LEI - US Department Of Agriculture (USDA)|
|ZHU, CHEN - Michigan State University|
|KUNZHE, DONG - Oak Ridge Institute For Science And Education (ORISE)|
Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2019
Publication Date: 7/15/2019
Citation: Zhang, H., Zhang, L., Zhu, C., Kunzhe, D., Heidari, M. 2019. Marek's Disease-vaccines induced differential expression of microRNAs in the primary lymphoid organ of bursa at 26th day post inoculation. Poultry Science Association Meeting Abstract. 98(E-Supplement 1):97.
Technical Abstract: MicroRNAs (miRNAs) are small noncoding RNAs, which are typically 17-24 nucleotides in length. Recent studies showed this epigenetic factor, miRNAs, along with long non-coding RNAs, drives phenotypic changes in both immunity and malignant cell development. MicroRNA alters the properties and behavior of cells through regulating gene expression and the accessibility of chromatin that determines cell fate. Those reports also showed cancer vaccine induces epigenetic changes. Our previous studies demonstrated that two highly inbred lines of White leghorns convey drastically different protective efficacy in response to Marek’s disease (MD) vaccines, as such HVT and CVI99/Rispens, against vv+ MD virus challenge. This study was designed to profile miRNAs present in the primary lymphoid organ, bursa, of the two inbred lines of chickens at 26th day post vaccination and to identify differentially expressed miRNAs induced by the vaccines. Chicks sampled from both MD-resistant line 63 and susceptible line 72 were inoculated on the day of hatch at a uniform dose of 2,000 FPU per bird for HVT or Rispens. A non-vaccinated control group was also included per line under the same conditions. Bursa tissues were taken on the 26th day post hatch from all treatment groups including the controls. Total RNA samples were extracted and subjected to small RNA sequencing on an Illumina HiSeq 4000 system. The miRDeep* package (v3.8) was run to identify miRNAs from the sequencing data. A total of 829 unique miRNAs was identified from all the treatment groups, including 621 novel miRNAs. HVT induced 4 and zero while Rispens induced 14 and 1 differentially expressed miRNAs in the line 63 and 72 birds, respectively. Within line, Rispens induced 29 and 3 differentially expressed miRNAs over HVT in lines 63 and 72, respectively; between the lines, Rispens induced 32 differentially expressed miRNAs in line 63 over line 72, and HVT induced 35, in line 63 over line 72. These results suggest CVI988/Rispens induced a stronger response in line 63 birds than in the line 72 birds. Pathways depicting target genes of the differentially expressed miRNAs will be discussed.