|CHAPPELL, LEN - Georgia Poultry Laboratory Network|
|KILLIAN, MARYLEA - Animal And Plant Health Inspection Service (APHIS)|
Submitted to: Animal Influenza Virus
Publication Type: Book / Chapter
Publication Acceptance Date: 5/1/2019
Publication Date: 3/27/2020
Citation: Spackman, E., Chappell, L., Killian, M. 2020. Detection of influenza A antibodies in avian serum samples by ELISA. In: Spackman, E., editor. Animal Influenza Virus Methods and Protocols. 3rd Edition. Methods in Molecular Biology (vol 2123). New York, NY: Humana. p. 151-167. https://doi.org/10.1007/978-1-4939-0758-8_14.
Technical Abstract: Enzyme-linked immunosorbent assays(ELISA) are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive they provide a ready to use assay with good quality control. Numerous sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation and troubleshooting are discussed.