Submitted to: Animal Influenza Virus
Publication Type: Book / Chapter
Publication Acceptance Date: 5/1/2019
Publication Date: 3/27/2020
Citation: Spackman, E., Lee, S.A. 2020. Avian influenza virus RNA extraction. In: Spackman, E., Editor. Animal Influenza Virus Methods and Protocols. 3rd Edition. Methods in Molecular Biology, volume 2123. New York, NY: Humana. p. 123-135. doi:https://doi.org/10.1007/978-1-0716-0346-8_10.
Technical Abstract: The efficient extraction and purification of viral RNA is critical for down-stream molecular applications such as the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds. Samples can generally be divided into two types; enriched (e.g. virus stocks) or non-enriched (e.g. clinical). Clinical samples, which may be tissues or swab material, are the most difficult to process due to the complex sample composition and possibly low virus titers. In this chapter, two well established procedures for the isolation of AI virus RNA from common clinical specimen types and enriched virus stocks will be presented.