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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #360028

Research Project: Ecology and Detection of Human Pathogens in the Produce Production Continuum

Location: Produce Safety and Microbiology Research

Title: Quantitating Shiga toxins produced by environmental E. coli

Author
item Silva, Christopher - Chris

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Other
Publication Acceptance Date: 12/21/2018
Publication Date: 2/6/2019
Citation: Silva, C.J., Lee, B.G., Yambao, J.C., Erickson-Beltran, M.L., Quinones B. 2019. Quantitating Shiga toxins produced by environmental E. coli. Cover, Journal of Agricultural and Food Chemistry. 67(5). February 6, 2019.

Interpretive Summary: N/A

Technical Abstract: Shiga toxin-producing Escherichia coli (STEC) were isolated from different environments in three California counties. Expression of Shiga toxins was induced by nutrient depletion. Mass spectrometry was used to identify and quantitate the Shiga toxins present in the culture supernatants. These STEC produced Shiga toxins over a fifty-fold range.