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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Publications at this Location » Publication #359327
Title: Selected lipid-based transfection reagents activate NF-kB and MAP kinases signaling pathways, induced cytokines mRNA expression in human THP-1 macrophage

Author
item CHOE, UYORY - University Of Maryland
item YU, LIANGLI - University Of Maryland
item Wang, Thomas - Tom

Submitted to: Analytical Biochemistry: Methods in the Biological Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/21/2019
Publication Date: 5/15/2019
Citation: Choe, U., Yu, L., Wang, T.T. 2019. Selected lipid-based transfection reagents activate NF-kB and MAP kinases signaling pathways, induced cytokines mRNA expression in human THP-1 macrophage. Analytical Biochemistry: Methods in the Biological Sciences. 573:73-76. https://doi.org/10.1016/j.ab.2019.02.018.
DOI: https://doi.org/10.1016/j.ab.2019.02.018

Interpretive Summary: Transfection is a common technique that introduces nucleic acid into mammalian cells to study, assess the biological effect of genes. In the present study, we evaluated, under transient transfection conditions, five different cationic lipid-based transfection reagents on the activation of NF-'B, MAP kinases signaling pathways and induction of cytokines expression. We found that the reagents studied differentially regulated the NF-'B and the MAP kinases signaling pathways in the human THP-1 macrophage. Additionally, mRNA expression levels of the cytokines, IL-1ß and TNF-a in THP-1 macrophage were also induced by selected test reagents. Hence, careful selection of cationic lipid-based transfection reagent for transient transfection is warranted, especially in studies of gene expression and function mediated through NF-kB- and MAP kinases-mediated pathways. This study provides novel information on commonly used reagents that will benefit both basic and translational scientists studying gene regulation.

Technical Abstract: In the present study, we evaluated, under transient transfection conditions, five different cationic lipid-based transfection reagents on the activation of NF-kB, MAP kinases signaling pathways and induction of cytokines expression. We found that the reagents studied differentially regulated the NF-kB and the MAP kinases signaling pathways in the human THP-1 macrophage. Additionally, mRNA expression levels of the cytokines, IL-1ß and TNF-a in THP-1 macrophage were also induced by selected test reagents. Hence, the careful selection of cationic lipid-based transfection reagent for transient transfection is warranted, especially in studies of gene expression and function mediated through NF-kB- and MAP kinases-mediated pathways.