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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Publications at this Location » Publication #356342
Research Project: Biting Arthropod Surveillance and Control

Location: Mosquito and Fly Research

Title: Assessment of selected Saudi and Yemeni Plants for Insecticidal Activities against the yellow fever mosquito Aedes aegypti (L.), and LC MS/MS and GC/MS analysis of bioactive extracts

Author
item AL-MASSARANI, SHAZA - King Saud University
item EL-SHAIBANY, AMINA - Sana'A University
item Tabanca, Nurhayat
item ALI, ABBAS - University Of Mississippi
item ESTEP, ALDEN - Navy Entomology Center Of Excellence, Cmave Detachment
item Becnel, James
item GOGER, FAITH - Anadolu Universtiy
item DEMIRCI, BETUL - Anadolu Universtiy
item EL-GAMAL, ALI - King Saud University
item BASER, K. HUSNU CAN - Near East University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/3/2019
Publication Date: 7/3/2019
Citation: Al-Massarani, S., El-Shaibany, A., Tabanca, N., Ali, A., Estep, A.S., Becnel, J.J., Goger, F., Demirci, B., El-Gamal, A., Baser, K. 2019. Assessment of selected Saudi and Yemeni Plants for Insecticidal Activities against the yellow fever mosquito Aedes aegypti (L.), and LC MS/MS and GC/MS analysis of bioactive extracts. Meeting Abstract. https://doi.org/10.1016/j.jsps.2019.07.001.
DOI: https://doi.org/10.1016/j.jsps.2019.07.001

Interpretive Summary: Abstract only.

Technical Abstract: Plant-based organic pesticides offer an effective, degradable, environmentally friendly and cheaper alternative to conventional synthetic pesticides. Saudi Arabia and Yemen are characterized by wide distinction of their flora due to climate and height differences among different areas. As part of our continuing investigation of native medicinal plants for interesting biological activities, thirty-three plants, obtained from diverse areas in Saudi Arabia and Yemen, were screened for insecticidal activity against the mosquito vector Aedes aegypti (L.). Out of the 59 tested organic extracts, Hyssopus officinalis, Nigella sativa, Ocimum tenuiflorum, Saussurea lappa and Taraxacum officinale produced over 80% mortality against adult female Ae. aegypti at 5 'g/mosquito and only one extract Aloe perryi showed 100% mortality against 1st instar Ae. aegypti larvae at 31.25 ppm. All active fractions were processed for bioactive compounds identification using LC-MS/MS and/or headspace SPME-GC/MS analysis. Careful examination of the mass spectra and detailed interpretation of the fragmentation pattern allowed the identification of tens of interesting biologically active secondary metabolites. Some compounds such as caffeic and quinic acid and their esters, 3- and 5- caffeoyl and 4, 5-dicaffeoylquinic acids were detected in most of the analyzed fractions. Additionally, luteolin, its glycoside and luteolin glucuronide and diglucuronide were also identified as bioactive compounds in several HPLC fractions. Volatile ketone, 6-methyl-5-hepten-2-one was identified from the A. perryi n-hexane fraction as a major compound. With the aid of bioguided-directed isolation and purification, our main future target will be obtaining highly active, safe and naturally-derived insecticides from the endemic plants of the Arabian Peninsula.