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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #356056

Research Project: Improvement of Barley and Oat for Enhanced Productivity, Quality, and Stress Resistance

Location: Small Grains and Potato Germplasm Research

Title: Mapping of crown rust resistance gene Pc53 in oat (Avena sativa)

Author
item YIMER, BELAYNEH - Orise Fellow
item Bonman, John
item Esvelt Klos, Kathy

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/29/2018
Publication Date: 12/26/2018
Citation: Yimer, B.A., Bonman, J.M., Esvelt Klos, K.L. 2018. Mapping of crown rust resistance gene Pc53 in oat (Avena sativa). PLoS One. 13:e0209105. https://doi.org/10.1371/journal.pone.0209105.
DOI: https://doi.org/10.1371/journal.pone.0209105

Interpretive Summary: Crown rust causes significant yield reduction in oats. Use of resistant cultivars is economically feasible and environmentally friendly way of managing the disease. To develop a resistant oat cultivar it is imperative to breed cultivars containing genes such as Pc53. Hence, determining the location of Pc53 on the oat chromosome improves the accuracy and speed of developing oat cultivars that are resistant to crown rust, and thereby contributes towards sustainable management of crown rust in oats.

Technical Abstract: Crown rust disease caused by the fungus Puccinia coronata f. sp. avenae (Pca) is a major production constraint of oat in North America, Europe and Australia. There are over 100 genes effective against one or more Pca races, but only a handful of seedling resistance (Pc) genes have been mapped to a known chromosomal location. The goal of the present study was to use linkage mapping to identify the genomic location of the Pc53 gene, and to produce a list of linked SNPS with potential as molecular markers for marker assisted breeding. The Pc53 gene was placed on the linkage group Mrg08 at 82.4 cM using F5-derived recombinant inbred lines (RILs) from a cross between the Pc53 carrier 6-112-1-15 and the susceptible cultivar Otana. The map location was validated using RILs from a cross between 6-112-1-15 and the Pc50 differential line. Single nucleotide polymorphism marker GMI_ES01_c28412_66 was the closest to Pc53. A major seedling resistance gene ‘PcKM’ and QTL QcC.Core.08.1, QCr.Core.08.2, QCr.Core.08.3 and QCr.cdl9-12D were previously reported on Mrg08. QPc.Core.08.1 and PcKM were mapped to within 1 cM of Pc53; but previous virulence studies have indicated separate identities. The chromosomal location of Pc53 and SNPs linked with it will facilitate the utilization of Pc53 in oat breeding programs.