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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #356011

Research Project: Intervention Strategies to Predict, Prevent and Control Disease Outbreaks Caused by Emerging Strains of Virulent Newcastle Disease Viruses

Location: Exotic & Emerging Avian Viral Diseases Research

Title: First genome sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

Author
item Ababneh, M. - Jordan University Of Science & Technology
item Ferreira, H.l. - Orise Fellow
item Khalifeh, M. - Jordan University Of Science & Technology
item Suarez, David
item Afonso, Claudio

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/5/2018
Publication Date: 12/13/2018
Citation: Ababneh, M., Ferreira, H., Khalifeh, M., Suarez, D.L., Afonso, C.L. 2018. First genome sequence of Newcastle Disease Virus of Genotype VIIi from Jordan. Microbiology Resource Announcements. 5(23):e01136-18. https://doi.org/10.1128/MRA.01136-18.
DOI: https://doi.org/10.1128/MRA.01136-18

Interpretive Summary: Newcastle disease is a significant disease of poultry and is a cause of significant concern for the US due to trade restrictions and to significant economic losses associated with the presence of the virus in poultry. The virulent forms of the virus are considered select agents and obtaining epidemiological information is important to trace the origins of outbreaks. The first complete genomic sequence from a virulent Newcastle disease virus present in 2018 in Jordan is presented here. The information from the complete genome will facilitate the epidemiological tracking of the virus and movement across the world.

Technical Abstract: Newcastle disease virus (NDV) was detected by RT-PCR from chicken RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained using MiSeq (Illumina) sequencing of total RNA obtained directly from the clinical sample. Phylogenetic analysis of the complete genome classified the isolate as class II sub-genotype VIIi.