|ABABNEH, M. - Jordan University Of Science & Technology|
|FERREIRA, H.L. - Orise Fellow|
|KHALIFEH, M. - Jordan University Of Science & Technology|
Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/5/2018
Publication Date: 12/13/2018
Citation: Ababneh, M., Ferreira, H., Khalifeh, M., Suarez, D.L., Afonso, C.L. 2018. First genome sequence of Newcastle Disease Virus of Genotype VIIi from Jordan. Microbiology Resource Announcements. 5(23):e01136-18. https://doi.org/10.1128/MRA.01136-18.
Interpretive Summary: Newcastle disease is a significant disease of poultry and is a cause of significant concern for the US due to trade restrictions and to significant economic losses associated with the presence of the virus in poultry. The virulent forms of the virus are considered select agents and obtaining epidemiological information is important to trace the origins of outbreaks. The first complete genomic sequence from a virulent Newcastle disease virus present in 2018 in Jordan is presented here. The information from the complete genome will facilitate the epidemiological tracking of the virus and movement across the world.
Technical Abstract: Newcastle disease virus (NDV) was detected by RT-PCR from chicken RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained using MiSeq (Illumina) sequencing of total RNA obtained directly from the clinical sample. Phylogenetic analysis of the complete genome classified the isolate as class II sub-genotype VIIi.