Submitted to: Chromatography Today
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/14/2018
Publication Date: 6/13/2018
Citation: Byrdwell, W.C. 2018. Blue jacaranda seed oil analyzed using comprehensive 2D-LC with quadruple parallel MS (LC2MS4). Journal of Chromatography. 56-64.
Interpretive Summary: We report a new type of analysis in which two separation techniques (liquid chromatography) are combined with nine different detectors for a thorough analysis of blue jacaranda seed oil and its fat-soluble vitamins (tocopherols). We report for the first time the presence and amounts of alpha-tocopherol and gamma-tocopherol (vitamin E), and also the compositions of diacylglycerols and triacylglylcerols (triglycerides) in extracts of blue jacaranda seeds. We use a new technique that changes the paradigm of how two-dimensional liquid chromatography is done. In the first dimension, we identify all molecules and count how much of each is present. In the second dimension we separate molecules that have the same masses, but are arranged differently (isomers). We identify trans fats, and show how they are arranged in the oil to determine how bioavailable they are. The blue jacaranda is used as an example, because little is known about its oil composition, but the technique is applicable to every kind of fat and oil.
Technical Abstract: We report 2D-LC in which two mass spectrometers in parallel plus four other detectors (UV, FLD, CAD, and ELSD) are used to monitor the first dimension (1D), while two more mass spectrometers in parallel plus a UV detector are used to monitor the second dimension (2D). LC1MS2 x LC1MS2 = LC2MS4 was employed to identify and quantify tocopherols, diacylglycerols (DAGs), and triacylglycerols (TAGs) in Jacaranda mimosifolia seed oil for the first time. Non-aqueous reversed-phase (NARP) HPLC in the 1D was coupled to ESI-MS and APCI-MS employing selected ion monitoring and selected reaction monitoring (SRM). We found 591.0 ± 13.5 ug/g of alpha-tocopherol and 517.6 ± 7.8 ug/g of gamma-tocopherol in JMSO by SRM APCI-MS, and report the percent relative compositions of DAGs and TAGs. We used a lab-made silver-ion column for UHPLC in the 2D coupled to APPI-MS and ESI-MS to identify cis/trans isomers and regioisomers.