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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Nutrient Data Laboratory » Research » Publications at this Location » Publication #351948

Research Project: USDA National Nutrient Databank for Food Composition

Location: Nutrient Data Laboratory

Title: 10-Hydroxy-trans-2-decenoic acid attenuates angiotensin II-induced inflammatory responses in rat vascular smooth muscle cells

Author
item Qingshuang, Cai - Anhui Agricultural University
item Shanming, Ji - Anhui Agricultural University
item Yue, Sun - Anhui Agricultural University
item Linsheng, Yu - Anhui Agricultural University
item Wu, Xianli
item Zhongwen, Xie - Anhui Agricultural University

Submitted to: Journal of Functional Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/16/2018
Publication Date: 4/21/2018
Citation: Qingshuang, C., Shanming, J., Yue, S., Linsheng, Y., Wu, X., Zhongwen, X. 2018. 10-Hydroxy-trans-2-decenoic acid attenuates angiotensin II-induced inflammatory responses in rat vascular smooth muscle cells. Journal of Functional Foods. 45, 298-305. 10.1016/j.ff.2018.04.034.
DOI: https://doi.org/10.1016/j.ff.2018.04.034

Interpretive Summary: 10-Hydroxy-trans-2-decenoic acid (10-HDA) is a major medium chain fatty acid in royal jelly. In this study, the effects of 10-HDA in attenuating angiotensin II (Ang II)-induced vascular inflammatory responses and the underlying molecular mechanisms were investigated in the rat vascular smooth muscle cells (VSMCs). The rat VSMCs were pretreated with 10-HDA, followed by incubation with Ang II. 10-HDA significantly reduced the productions of inflammatory cytokines and reactive oxygen species (ROS) production induced by Ang II. 10-HAD was found to down regulate two important pro-inflammatory signaling pathways. 10-HDA also reduced the reactive oxygen species production and the NADPH oxidase 4 (NOX4) expression. Further mechanistic studies revealed that 10-HDA also reduced elevated expression of other factors related to chronic inflammation. The findings suggested 10-HDA to be a promising nature product in preventing vascular inflammation.

Technical Abstract: 10-Hydroxy-trans-2-decenoic acid (10-HDA) is a major medium chain fatty acid in royal jelly. In this study, the effects of 10-HDA in modulating angiotensin II (Ang II)-induced vascular inflammatory responses and the underlying molecular mechanisms were investigated in the rat vascular smooth muscle cells (VSMCs). The rat VSMCs were pretreated with 10-HDA (250 µM, 500 µM, 1000 µM), followed by incubation with Ang II (100 nM). 10-HDA significantly reduced the productions of inflammatory cytokines IL-6 and CRP induced by Ang II, through downregulating the nuclear factor kappa B (NF-'B) and mitogen-activated protein kinase (MAPK) signaling pathways. 10-HDA also attenuated the Ang II-stimulated reactive oxygen species (ROS) production and the NADPH oxidase 4 (NOX4) expression. Further mechanistic studies showed that 10-HDA reduced the elevated abundances of the angiotensin II type 1 receptor (AT1R), and inhibited cAMP-response element-binding protein (CREB) binding to the cAMP-response element (CRE) site in the IL-6 promoter region. 10-HDA effectively attenuated Ang II-induced inflammatory responses and ROS production via regulating multiple steps in NF-'B and MAPK signaling pathways. The findings supported 10-HDA to be a potential bioactive dietary component in preventing vascular inflammation.