|Goraichuk, Iryna - Consultant|
|Dimitrov, Kiril - Consultant|
|Sharma, Poonam - Food Safety Inspection Service (FSIS)|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2018
Publication Date: N/A
Technical Abstract: Four viral isolates from Rockhopper Penguins were previously identified as members of a novel avian paramyxovirus serotype 10 (APMV-10). Whole genome random next-generation sequencing was performed and phylogenetic analysis showed that the isolates were most closely related to APMV-2 and APMV-8. Intracerebral pathogenicity index test indicated that the viruses are not virulent for chickens. One of the isolated viruses, APMV10-Rock-Hopper-Penguin-Falkland-Islands-539-2007, was further characterized in vivo. Three groups (I, II and III, n = 5 each) of 3-week-old SPF chickens were inoculated oculonasally with 102, 104, 106 EID50 of the virus per bird, respectively. At 2 days post inoculation (dpi) three additional SPF chickens were placed into each group to evaluate virus transmission. Serum samples were collected at 14 dpi. No chickens from group I seroconverted after inoculation with APMV-10, one and five directly inoculated chickens from groups II and III developed anti-APMV-10 antibodies as demonstrated by HI. No chickens showed any apparent clinical signs after inoculation with APMV-10. At 14 dpi the three contact chickens from group I and all inoculated chickens from groups II and III were challenged oculonasally with 106 EID50 per bird of virulent NDV-California-2002. Cloacal and oropharyngeal swabs were collected at 2 and 4 days post challenge (dpc). Clinical signs with 100% mortality were observed in all chickens after challenge with the virulent NDV strain. The APMV-10 virus was able to replicate in chickens at higher challenge doses, but without causing any clinical signs. Also, no cross protection against virulent NDV challenge was observed.