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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #350053

Research Project: Improved Analytical Technologies for Detection of Foodborne Toxins and Their Metabolites

Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Development of antibodies for N-(1-deoxy-D-fructos-1-yl) fumonisin B1 and cross-reaction with modified fumonisins

item Maragos, Chris
item Sieve, Kristal
item Busman, Mark

Submitted to: World Mycotoxin Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/19/2018
Publication Date: 6/11/2019
Citation: Maragos, C.M., Sieve, K.K., Busman, M. 2019. Development of antibodies for N-(1-deoxy-D-fructos-1-yl) fumonisin B1 and cross-reaction with modified fumonisins. World Mycotoxin Journal. 11:493-502.

Interpretive Summary: Fumonisins are a group of toxins produced by fungi (mycotoxins) that are routinely found worldwide in commodities such as maize. Monitoring for these toxins is conducted in order to effectively divert infested commodities from the human food and animal feed supplies. However, such monitoring is complicated by the fact that certain forms of these toxins are not detected by many of the common ways that commodities are monitored. These so-called "masked" toxins are of indeterminate hazard and, because of this, rapid methods for their detection are desired. ARS scientists in Peoria, Illinois, have developed an antibody-based screening assay that detects several of the masked forms of the fumonisin mycotoxins. The assay will be a useful tool for determining the extent to which the masked fumonisins are present in maize and whether or not such forms represent an additional hazard to human or animal health.

Technical Abstract: Fumonisins are a group of mycotoxins that are routinely found worldwide in commodities such as maize. The group, which has many members, is generally characterized by the presence of one or more tricarballylic acid groups esterified to a long carbon backbone. The diversity of this group of toxins is further augmented by their ability to interact with matrix components non-covalently and to form covalent products with matrix constituents such as carbohydrates and proteins. Covalent modifications to the toxins make it more difficult to assess the total amounts that may be present in a commodity. We developed monoclonal antibodies (Mabs) against a known product of the reaction of fumonisin B1 (FB1) with glucose: N-(1-Deoxy-D-fructos-1-yl) fumonisin B1 (NDFrc-FB1). Similar reactions were used to produce fructosyl-analogs of fumonisins B2 and B3, as well as galactose, maltose, and rhamnose analogs of FB1. These analogs were tested in a competitive indirect ELISA for cross-reactivity towards one of the developed antibodies (Mab 213221). All of the carbohydrate analogs cross-reacted with the Mab, at levels ranging from 75% (the FB3 analog derived from D-glucose) to 181% (the FB1 analog derived from maltose). These results suggested the assay was capable of binding to a wide variety of fumonisin-carbohydrate derivatives. The same antibody was incorporated into an immunoaffinity column that was used to isolate modified fumonisins from a sample of naturally contaminated maize. These results demonstrate the potential to isolate and detect modified fumonisins and will facilitate efforts to determine the prevalence of these compounds in maize.