Location: Bioenergy ResearchTitle: Production of acetone butanol ethanol (ABE) from Physaria fendleri press cake using Clostridium beijerinckii
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/12/2017
Publication Date: N/A
Technical Abstract: Physaria fendleri (PF) is a member of the mustard family that is of current interest for commercial production as an oil crop because its oil has industrial uses. Oil production costs are highly sensitive to finding a use for the press cake left over following oil extraction. In this study, PF press cake is evaluated as a feedstock for butanol production because of its high carbohydrate content. To extract the carbohydates, 60 gL-1 PF was pretreated with 0.5% (v/v) sulfuric acid at 121 deg C for 1 h followed by cooling to 25 deg C and agitating the mixture at 200 rpm using a magnetic stir bar for 72 h. The resultant hydrolyzate contained 15.83 gL-1 total sugars. Then the hydrolyzate was fermented using Clostridium beijerinckii P260 and the culture produced 6.39 gL-1 ABE with a productivity of 0.18 gL-1h-1. These experiments suggested that enzymatic hydrolysis of PF after pretreatment was essential to release more sugars in the fermentation medium. Upon hydrolysis of 60 gL-1 PF with enzymes followed by fermentation provided with13.45 gL-1 ABE in the fermentation broth. Increasing PF concentration to 100 gL-1 followed by enzymatic hydrolysis resulted in the production of 19.22 gL-1 ABE with a productivity of 0.40 gL-1h-1. These results are comparable to ABE/butanol production observed from a control fermentation using glucose. On a routine basis not more than 18-20 gL-1 ABE is produced in batch reactors from glucose based medium. These results suggest that biobutanol should be explored further as a co-product for PF oil production.