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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sunflower and Plant Biology Research » Research » Publications at this Location » Publication #345447

Title: Genetic architecture of capitate glandular trichome density in florets of domesticated sunflower (Helianthus annuus L.)

item Gao, Qing-Ming
item KANE, NOLAN - University Of Colorado
item Hulke, Brent
item REINERT, STEPHAN - University Of Colorado
item POGODA, CLOE - University Of Colorado
item TITTES, SILAS - University Of Colorado
item Prasifka, Jarrad

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/18/2017
Publication Date: 1/9/2018
Citation: Gao, Q.M., Kane, N.C., Hulke, B.S., Reinert, S., Pogoda, C.S., Tittes, S., Prasifka, J.R. 2018. Genetic architecture of capitate glandular trichome density in florets of domesticated sunflower (Helianthus annuus L.). Frontiers in Plant Science.

Interpretive Summary: Plants produce chemicals that can sometimes act to deter or kill insects. There is evidence for their involvement in resistance to sunflower moth, a seed feeding pest of sunflower. The purpose of this paper is to describe how the flower parts that produce these chemicals are inherited in sunflower, using a plant population developed from a cross between one parent lacking these flower parts and chemicals and another parent that produces large quantities of them. Two significant mutations were found on different chromosomes, suggesting that the resistance to sunflower moth trait would be fairly simple to bring into any sunflower line through breeding. Known genes in the region of the two mutations are similar to genes that are known to have a function in developing these flower parts in other plants. This further validates our findings.

Technical Abstract: Capitate glandular trichomes (CGT), one type of glandular trichomes, are most common in Asteraceae species. Capitate glandular trichomes can produce various secondary metabolites such as sesquiterpene lactones (STLs) and provide durable resistance to insect pests. In sunflower, CGT-based host resistance is effective to combat the specialist pest, sunflower moth. However, the genetic basis of CGT density is not well understood in sunflower. In this study, we identified two major QTL controlling CGT density in sunflower florets by using a F4 mapping population derived from the cross HA 300 × RHA 464 with a genetic linkage map constructed from genotyping-by-sequencing data and composed of 2121 SNP markers. One major QTL is located on chromosome 5, which explained 11.61% of the observed phenotypic variation, and the second QTL is located on chromosome 6, which explained 14.06% of the observed phenotypic variation. The QTL effects and the association between CGT density and QTL support interval were confirmed in a validation population which included 39 sunflower inbred lines with diverse genetic backgrounds. We also identified two strong candidate genes in the QTL support intervals, and the functions of their orthologs in other plant species suggested their potential roles in regulating capitate glandular trichome density in sunflower. Our results provide valuable information to sunflower breeding community for developing host resistance to sunflower insect pests.