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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #343818

Research Project: Genetic Enhancement of Dry Bean Nutritional and Processing Qualities

Location: Sugarbeet and Bean Research

Title: QTL analysis of Fusarium root rot resistance in an Andean x Middle American common bean RIL population

Author
item WANG, WEIJIA - Michigan State University
item JACOBS, JANETTE - Michigan State University
item CHILVERS, MARTIN - Michigan State University
item MUKANKUSI, CLARE - International Center For Tropical Agriculture (CIAT)
item KELLY, JAMES - Michigan State University
item Cichy, Karen

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2018
Publication Date: 4/5/2018
Citation: Wang, W., Jacobs, J.L., Chilvers, M.I., Mukankusi, C.M., Kelly, J.D., Cichy, K.A. 2018. QTL analysis of Fusarium root rot resistance in an Andean x Middle American common bean RIL population. Crop Science. 58:1166-1180. doi:10.2135/cropsci2017.10.0608.
DOI: https://doi.org/10.2135/cropsci2017.10.0608

Interpretive Summary: Fusarium root rot (FRR) is the most serious soil borne disease in the U.S. FRR causes major yield losses especially in large seeded Andean beans, such as kidney beans that have little genetic resistance. Sources of FRR resistance of value to U.S. Andean bean breeding programs were identifed. Genomic regions associated with FRR resistance in both greenhouse screening to a specific virulent strain and under natural field FRR disease pressure were identified on three bean chromosome Pv02, Pv07 and Pv11. The identified quantitative trait loci are candidates for marker-assisted selection.

Technical Abstract: Aims Fusarium root rot (FRR) is a soil-borne disease that constrains common bean (Phaseolus vulgaris L.) production. FRR causal pathogens include clade 2 members of the Fusarium solani species complex. Here we characterize common bean reaction to four Fusarium species and identify genomic regions associated with resistance. Methods Four Fusarium species, F. brasiliense, F. cuneirostrum, F. solani sensu stricto, and F. oxysporum sensu lato were tested for virulence on two bean genotypes: CAL96 (Andean) and MLB-49-89A (Middle American). The RIL population of CAL96 x MLB-49-89A was phenotyped in a greenhouse for FRR resistance with the F. brasilense isolate and screened in the field under natural FRR disease pressure. QTL mapping was conducted on an map developed with SNP markers. Results Tested Fusarium species isolates varied in virulence. A total of 17 QTL related to FRR resistance and root/shoot biomass were identified in the RIL population. QTL associated with disease severity score (DS) in both greenhouse and field co-localized on chromosome Pv02. Root and shoot weight QTL from field and greenhouse screens also co-localized on Pv07 and Pv11. Conclusions FRR related QTL identified that are strong candidates for marker-assisted selection.