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Title: Registration of the LouAlp (Louise/Alpowa) wheat recombinant inbred line mapping population

item MARTINEZ, SHANTEL - Washington State University
item Thompson, Alison
item WEN, NUAN - Washington State University
item Murphy, Lesley
item SANGUINET, KAREN - Washington State University
item Steber, Camille
item Garland-Campbell, Kimberly

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/15/2017
Publication Date: 4/26/2018
Citation: Martinez, S.A., Thompson, A.L., Wen, N., Murphy, L.R., Sanguinet, K., Steber, C.M., Garland Campbell, K.A. 2018. Registration of the LouAlp (Louise/Alpowa) wheat recombinant inbred line mapping population. Journal of Plant Registrations. https://doi:10.3198/jpr2017.08.0053crmp.

Interpretive Summary: Although wheat is more drought tolerant than other major US crops, increasing genetic drought tolerance is critical for Western US wheat production. The cultivar Louise exhibits high yield both in regions with high and low soil moisture. Alpowa grows more slowly than Louise, then grows faster as it transitions into flowering and enters grain filling thereby supporting higher yields. Our goal was to develop a recombinant inbred line (RIL) population segregating for differences in stress resistance and for genes governing flowering time and development in PNW cultivars. The LouAlp (Louise x Alpowa) mapping population consists of 141 RILs, of which 132 RILs were used to create a genetic linkage map. Furthermore, different combinations of flowering time genes, Ppd-D1 and Vrn, had an impact on the growth stage. This population also shows no difference in the reduced height genes, Rht, but still show a wide variation of height in irrigated and non-irrigated systems. Since this population was derived from cultivars well-adapted in the inland Northwest, introgression of RILs with ideal phenotypes and genetics into breeding programs should be successful.

Technical Abstract: A mapping population was developed from the cross of soft white spring wheat (Triticum aestivum L.) cultivars ‘Louise’ and ‘Alpowa’ for use in investigating the genetic architecture of drought tolerance in the Pacific Northwest. The LouAlp (Louise x Alpowa) (Reg. No. MP-__, NSL_____)recombinant inbred line mapping population was developed through single seed descent from the F2 generation to the F5 generation. The population consists of 141 F2:5 RILs, of which 132 were used to construct the genetic linkage map. The 32 linkages groups included 881 single nucleotide polymorphic marker from the Illumina iSelect SNP assay, and two single sequence repeat markers spanning all 21 chromosomes, except chromosomes 1D, 3D, and 4D. The LouAlp population was characterized for variation in agronomic traits, flowering time, and phenology. This population will be used for identification and introgression of multiple loci providing resistance to environmental stress such as drought, stripe rust, and heat stress.