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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #342889
Research Project: Ecology and Detection of Human Pathogens in the Produce Production Continuum

Location: Produce Safety and Microbiology Research

Title: Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai

Author
item NI, PEIEN - Shanghai Jiaotong University
item XU, QIAN - Shanghai Jiaotong University
item YIN, YUJIE - Shanghai Jiaotong University
item LIU, DANLEI - Shanghai Jiaotong University
item ZHANG, JYMEI - Guangdong Academy Of Agricultural Sciences
item WU, QIANPING - Guangdong Academy Of Agricultural Sciences
item Tian, Peng
item SHI, XIANMING - Shanghai Jiaotong University
item WANG, DAPENG - Shanghai Jiaotong University

Submitted to: Food Control
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/10/2017
Publication Date: 10/11/2017
Citation: Ni, P., Xu, Q., Yin, Y., Liu, D., Zhang, J., Wu, Q., Tian, P., Shi, X., Wang, D. 2017. Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai. Food Control. 85:269-275. https://doi.org/10.1016/j.foodcont.2017.10.009.
DOI: https://doi.org/10.1016/j.foodcont.2017.10.009

Interpretive Summary:

Technical Abstract: Salmonella is a major bacterial pathogen causing food-borne illness worldwide. Farm products, including raw meat, vegetable and dairy products, could be one of the important vehicles to transmit foodborne pathogens, which threatened to human health. The prevalence of Salmonella isolated from farm products in Shanghai was investigated and characterized in collaboration with researches in Shanghai Jiao Tong University. In this study, 344 samples were randomly collected from farmers' markets, supermarkets, milk factories and a slaughterhouse in Shanghai between March 2016 and February 2017. By using the method of Chinese National Standard (GB 4789.4-2010), 83 (24.1%) samples were positive for Salmonella including 42 (42.4%) of pork, 21 (41.2%) of chicken, 15 (31.3%) of beef, 3 (16.7%) of mutton, 1 (4.5%) of lettuce, 1(3.8%) of cucumber. Egg and raw milk samples were negative. Ninety strains were isolated from those positive samples. In the isolates, 12 types of serovar were identified including Enteritidis (n=35), Typhimurium (n=22), Derby (n=13), Anatum (n=5), Agona (n=4), Thompson(n=3), Indiana (n=3), Braenderup (n=1), Heidelberg (n=1), Infantis (n=1),Litchfield (n=1) and Manhattan (n=1). All isolates were characterized by multilocus sequence typing (MLST) and subtyped by pulsed-field gel electrophoresis (PFGE). Thirteen sequence types (ST) were identified, and ST11, ST34 and ST40 were the predominant sequence types. The sequence types had high correlation with serovar. The results of PFGE showed that 73 PFGE patterns were generated from those isolates. In conclusion, Salmonella contamination was common in farm products in Shanghai, and the diversity of the pathogen was high.