Location: Produce Safety and Microbiology ResearchTitle: Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai
|NI, PEIEN - Shanghai Jiaotong University|
|XU, QIAN - Shanghai Jiaotong University|
|YIN, YUJIE - Shanghai Jiaotong University|
|LIU, DANLEI - Shanghai Jiaotong University|
|ZHANG, JYMEI - Guangdong Academy Of Agricultural Sciences|
|WU, QIANPING - Guangdong Academy Of Agricultural Sciences|
|SHI, XIANMING - Shanghai Jiaotong University|
|WANG, DAPENG - Shanghai Jiaotong University|
Submitted to: Food Control
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/10/2017
Publication Date: 10/11/2017
Citation: Ni, P., Xu, Q., Yin, Y., Liu, D., Zhang, J., Wu, Q., Tian, P., Shi, X., Wang, D. 2017. Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai. Food Control. 85:269-275. https://doi.org/10.1016/j.foodcont.2017.10.009.
Technical Abstract: Salmonella is a major bacterial pathogen causing food-borne illness worldwide. Farm products, including raw meat, vegetable and dairy products, could be one of the important vehicles to transmit foodborne pathogens, which threatened to human health. The prevalence of Salmonella isolated from farm products in Shanghai was investigated and characterized in collaboration with researches in Shanghai Jiao Tong University. In this study, 344 samples were randomly collected from farmers' markets, supermarkets, milk factories and a slaughterhouse in Shanghai between March 2016 and February 2017. By using the method of Chinese National Standard (GB 4789.4-2010), 83 (24.1%) samples were positive for Salmonella including 42 (42.4%) of pork, 21 (41.2%) of chicken, 15 (31.3%) of beef, 3 (16.7%) of mutton, 1 (4.5%) of lettuce, 1(3.8%) of cucumber. Egg and raw milk samples were negative. Ninety strains were isolated from those positive samples. In the isolates, 12 types of serovar were identified including Enteritidis (n=35), Typhimurium (n=22), Derby (n=13), Anatum (n=5), Agona (n=4), Thompson(n=3), Indiana (n=3), Braenderup (n=1), Heidelberg (n=1), Infantis (n=1),Litchfield (n=1) and Manhattan (n=1). All isolates were characterized by multilocus sequence typing (MLST) and subtyped by pulsed-field gel electrophoresis (PFGE). Thirteen sequence types (ST) were identified, and ST11, ST34 and ST40 were the predominant sequence types. The sequence types had high correlation with serovar. The results of PFGE showed that 73 PFGE patterns were generated from those isolates. In conclusion, Salmonella contamination was common in farm products in Shanghai, and the diversity of the pathogen was high.