Location: National Peanut Research LaboratoryTitle: Differential Expression of R-genes to Associate Leaf Spot Resistance in Cultivated Peanut
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2017
Publication Date: N/A
Technical Abstract: Breeding for acceptable levels of Early (ELS) or Late Leaf Spot (LLS) resistance in cultivated peanut has been elusive due to extreme variability of plant response in the field and the proper combinations of resistance (R)-genes in any particular peanut line. R-genes have been shown to be involved in disease resistance in many important crop plants and may contribute to leaf spot resistance in peanuts. The goals of this research are to characterize gene-expression of candidate R-genes in peanut and to associate gene-expression profiles in susceptible (Florunner 458), medium resistance (Exp27-1516), and tolerant (GA12Y) runner-peanut genotypes. Out of a 209 gene targets, 110 potential R-genes were evaluated utilizing real-time PCR analysis from leaf RNAs extracted from ELS and LLS infected peanut plants. Analysis revealed 54 up- and 56 down-regulated R-genes when Florunner 458 was utilized to normalize real-time PCR data. Association of gene-expression profiles to disease resistance levels will facilitate the selection of peanut lines with higher disease resistance.